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Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/28
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(New page: {{:Team:Tokyo_Metropolitan/Header}} =2010/08/28= ==1：PCR == ＜Member＞<br /> Mariko, Hitomi ＜Sample＞<br /> ・E-coli (having BBa_I732901 plasmid) ＜Protocol＞<br /> See [htt...) |
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| - | See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol# | + | See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ] |
Revision as of 08:46, 4 September 2010
Contents |
2010/08/28
1：PCR
＜Member＞
Mariko, Hitomi
＜Sample＞
・E-coli (having BBa_I732901 plasmid)
＜Protocol＞
See Protocol 2
・Tube(higher temperature/lower temperature in annealing)
- Rfp (67.5℃/66.0)
- Terminator (66.0/63.0)
Total 4 tubes.
2：Plasmid extraction from E-coli
＜Member＞
Hitomi
＜Sample＞
・transformed E-coli (8/26)
- BBa_I13521
- BBa_B0010
- BBa_B0030
- BBa_J23110
- BBa_E0040
＜Protocol＞
SeeProtocol 6
3：DNA concentration measurement
＜Member＞
Hitomi
＜Sample＞
・PCR products
＜Protocol＞
- Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
- Fall in drops 1 on the measuring machine.
＜Result＞
| concentration | A320 | A260/A280 | A260/A230 | |
|---|---|---|---|---|
| RH | 565.0 | 20.24 | 1.723 | 1.175 |
| RL | 400.5 | 10.73 | 1.697 | 1.462 |
| TH | 356.0 | 8.47 | 1.622 | 1.201 |
| TL | 324.5 | 8.31 | 1.627 | 1.195 |
4：Electrophoresis
＜Member＞
Mariko, Hitomi
＜Sample＞
・PCR products
＜Protocol＞
SeeProtocol 8
＜Result＞
