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Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/07
From 2010.igem.org
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Mariko, easily | Mariko, easily | ||
Revision as of 12:25, 3 September 2010
2010/08/07
Experiment1：Making LB plate (20ml×20)
＜Member＞
Mariko, easily
＜Materials＞
・Yeast extract 2.0g
・Peptone 2.0g
・NaCl 2.0g
・Agerose 6.0g
・Ampcillim 0.4mg
・Dropped water 400ml
＜Protocol＞
1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, NaCl 2.0g and Agerose 6.0g.
2 Divided up 400ml solution into two Erlenmeyer flasks, and sealed with aluminum. Afterwards , started autoclave(121℃,20min)
3 After finished autoclave, in cleanbench, added 0.2ml Ampcillm to each of the solutions. Afterwards, divided solutions into twenty Petri dishes.
4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator.
