2010/08/07

1：Making LB plate (20ml×20)

＜Member＞
Mariko, easily

＜Materials＞
・Yeast extract 2.0g

・Peptone 2.0g

・NaCl 2.0g

・Agerose 6.0g

・Ampcillim 0.4mg

・Dropped water 400ml

＜Protocol＞
1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, NaCl 2.0g and Agerose 6.0g.

2 Divided up 400ml solution into two Erlenmeyer flasks, and sealed with aluminum. Afterwards , started autoclave(121℃,20min)

3 After finished autoclave, in cleanbench, added 0.2ml Ampcillm to each of the solutions. Afterwards, divided solutions into twenty Petri dishes.

4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator.