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Revision as of 12:53, 15 August 2010 (view source) Hitomi (Talk | contribs) (New page: {{:Team:Tokyo_Metropolitan/Header}} =2010/08/07= ==Experiment3：Making LB plate (20ml×20)== ＜Member＞Mariko, easily ＜Materials＞<br /> ・Yeast extract 2.0g ・Peptone 2.0g ...) ← Older edit		Revision as of 12:24, 3 September 2010 (view source) Hitomi (Talk | contribs) (→Experiment3：Making LB plate (20ml×20)) Newer edit →
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	＜Materials＞<br />		＜Materials＞<br />
	・Yeast extract 2.0g		・Yeast extract 2.0g

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Revision as of 12:24, 3 September 2010

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2010/08/07

Experiment1：Making LB plate (20ml×20)

＜Member＞

Mariko, easily

＜Materials＞
・Yeast extract 2.0g

・Peptone 2.0g

・NaCl 2.0g

・Agerose 6.0g

・Ampcillim 0.4mg

・Dropped water 400ml

＜Protocol＞
1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, NaCl 2.0g and Agerose 6.0g.

2 Divided up 400ml solution into two Erlenmeyer flasks, and sealed with aluminum. Afterwards , started autoclave(121℃,20min)

3 After finished autoclave, in cleanbench, added 0.2ml Ampcillm to each of the solutions. Afterwards, divided solutions into twenty Petri dishes.

4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator.