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Team:Caltech/Week 10
From 2010.igem.org
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==Monday 8/16== | ==Monday 8/16== | ||
| + | * Group meeting! | ||
| + | * Ran CPCR gels on L31, L34, L43, L58 & L71 | ||
| + | ** All negative. | ||
==Tuesday 8/17== | ==Tuesday 8/17== | ||
| + | * Took our team photo! | ||
| + | * Ran more CPCR of L58 & L81. | ||
| + | ** Gel was negative. | ||
| + | * Cultured HSP-LacI-GFP cells in LB + 1M glucose to try to up-regulate LacI repression of GFP production. Incubated cells at 37°C overnight. | ||
==Wednesday 8/18== | ==Wednesday 8/18== | ||
| + | * LacI appears fully repressed in L62 cells after overnight incubation at 37°C. | ||
| + | ** Will now spin down cells and resuspend in LB + IPTG to see if overnight incubation now activates GFP production, as predicted. | ||
| + | * Picked more CPCR colonies of L81 & L58 (x6) to try to find plastic production cells. | ||
| + | * Transformed L60 & L61. Plated on plates containing 50μL 50mM glucose (dried). | ||
| + | ** We hypothesize that the reason these ligations produced no colonies is due to leaky LacI repression, allowing a low level of lysis expression, eventually killing all the cells. Will see if there are cells on these plates tomorrow. | ||
| + | * Submitted our poster deign for printing for Sasha's SURF presentation tomorrow. | ||
==Thursday 8/19== | ==Thursday 8/19== | ||
Revision as of 01:32, 19 August 2010
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People
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 Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15
Monday 8/16
Tuesday 8/17
Wednesday 8/18
Thursday 8/19Friday 8/20
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