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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/13
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(New page: {{:Team:Tokyo_Metropolitan/Header}} ===Experiment:restriction enzyme digestion=== ====member:hiromi kanoko kazuki==== ===Materials=== *PCR products 50μl×3 *m buffer 15μl *BSA 1 15...)
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(New page: {{:Team:Tokyo_Metropolitan/Header}} ===Experiment:restriction enzyme digestion=== ====member:hiromi kanoko kazuki==== ===Materials=== *PCR products 50μl×3 *m buffer 15μl *BSA 1 15...)
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Revision as of 13:46, 13 August 2010
Contents |
Experiment:restriction enzyme digestion
member:hiromi kanoko kazuki
Materials
- PCR products 50μl×3
- m buffer 15μl
- BSA 1 15μl
- Xba1 3μl
- SPE1 3μl
Equipment
- pipet
- pipet tip
Procedure
1)mix materials : m buffer(5μl) , BSA1(5μl) , Xba1(1μl) and SPE1 (1μl) to PCR products(50μl). Repeat this process 3 times , for 3 PCR products.
2)incubation(37℃).
