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Team:Caltech/Week 9
From 2010.igem.org
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==Monday 8/9== | ==Monday 8/9== | ||
| + | * Group meeting! | ||
| + | * Re-ran two gels of CPCR results | ||
| + | ** Successful: 55-5, 56-1/-2/-3, 57-1/-2/-3, 54-5/-6 | ||
| + | ** Prepped DNA for use in digestions tomorrow | ||
==Tuesday 8/10== | ==Tuesday 8/10== | ||
| + | * Digested DNA for ligations: | ||
| + | ** L31: L17 + <partinfo>K215000</partinfo> + Kan | ||
| + | ** L32: L19 + L20 + Tet | ||
| + | ** L33: L19 + L18 + Tet | ||
| + | ** L34: L17 + L21 + Amp | ||
| + | ** L35: L18 + L21 + Amp | ||
| + | ** L41: L26 + <partinfo>B0015</partinfo> + Tet | ||
| + | ** L49: HSP + L40 + Tet | ||
| + | ** L60: <partinfo>K215000</partinfo> + L57 + Tet | ||
| + | ** L61: <partinfo>K215000</partinfo> + L56 + Tet | ||
| + | ** L63: L29 + L20 + Amp | ||
| + | ** L64: L27 + L20 + Amp | ||
| + | * Prepped 37-1, 54-5/-6, 55-5, 56, 57 DNA | ||
| + | * Started CPCR of L36, L38 | ||
| + | ** All colonies failed | ||
| + | * Started liquid culture of <partinfo>I15008</partinfo> | ||
==Wednesday 8/11== | ==Wednesday 8/11== | ||
| + | * <partinfo>I15008</partinfo> culture did not grow; will re-pick more tomorrow. | ||
| + | * L43 plate had no colonies; will redo L43 tomorrow. | ||
| + | * Began CPCR of all successful ligations. | ||
==Thursday 8/12== | ==Thursday 8/12== | ||
Revision as of 22:59, 11 August 2010
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People
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 Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15
Monday 8/9
Tuesday 8/10
Wednesday 8/11
Thursday 8/12Friday 8/13Weekend 8/14-8/15
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