Team:Caltech/Week 9

From 2010.igem.org

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==Monday 8/9==
==Monday 8/9==
 +
* Group meeting!
 +
* Re-ran two gels of CPCR results
 +
** Successful: 55-5, 56-1/-2/-3, 57-1/-2/-3, 54-5/-6
 +
** Prepped DNA for use in digestions tomorrow
==Tuesday 8/10==
==Tuesday 8/10==
 +
* Digested DNA for ligations:
 +
** L31: L17 + <partinfo>K215000</partinfo> + Kan
 +
** L32: L19 + L20 + Tet
 +
** L33: L19 + L18 + Tet
 +
** L34: L17 + L21 + Amp
 +
** L35: L18 + L21 + Amp
 +
** L41: L26 + <partinfo>B0015</partinfo> + Tet
 +
** L49: HSP + L40 + Tet
 +
** L60: <partinfo>K215000</partinfo> + L57 + Tet
 +
** L61: <partinfo>K215000</partinfo> + L56 + Tet
 +
** L63: L29 + L20 + Amp
 +
** L64: L27 + L20 + Amp
 +
* Prepped 37-1, 54-5/-6, 55-5, 56, 57 DNA
 +
* Started CPCR of L36, L38
 +
** All colonies failed
 +
* Started liquid culture of <partinfo>I15008</partinfo>
==Wednesday 8/11==
==Wednesday 8/11==
 +
* <partinfo>I15008</partinfo> culture did not grow; will re-pick more tomorrow.
 +
* L43 plate had no colonies; will redo L43 tomorrow.
 +
* Began CPCR of all successful ligations.
==Thursday 8/12==
==Thursday 8/12==

Revision as of 22:59, 11 August 2010


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Monday 8/9

  • Group meeting!
  • Re-ran two gels of CPCR results
    • Successful: 55-5, 56-1/-2/-3, 57-1/-2/-3, 54-5/-6
    • Prepped DNA for use in digestions tomorrow

Tuesday 8/10

  • Digested DNA for ligations:
    • L31: L17 + <partinfo>K215000</partinfo> + Kan
    • L32: L19 + L20 + Tet
    • L33: L19 + L18 + Tet
    • L34: L17 + L21 + Amp
    • L35: L18 + L21 + Amp
    • L41: L26 + <partinfo>B0015</partinfo> + Tet
    • L49: HSP + L40 + Tet
    • L60: <partinfo>K215000</partinfo> + L57 + Tet
    • L61: <partinfo>K215000</partinfo> + L56 + Tet
    • L63: L29 + L20 + Amp
    • L64: L27 + L20 + Amp
  • Prepped 37-1, 54-5/-6, 55-5, 56, 57 DNA
  • Started CPCR of L36, L38
    • All colonies failed
  • Started liquid culture of <partinfo>I15008</partinfo>

Wednesday 8/11

  • <partinfo>I15008</partinfo> culture did not grow; will re-pick more tomorrow.
  • L43 plate had no colonies; will redo L43 tomorrow.
  • Began CPCR of all successful ligations.

Thursday 8/12

Friday 8/13

Weekend 8/14-8/15

 
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