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BIOTEC Dresden/Notepad/23 July 2010
From 2010.igem.org
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We picked two colonies for each the following parts | We picked two colonies for each the following parts | ||
| - | 2a, 3a, 3b, 6a, 6b, 9a, 9b, 11a, 11b, 14a, 14b, 15a, 15b, 20a, 20b, 21a, 21b, 27a, 27b, 28a, 28b, 29a, 29b, 30a, 30b, 31a, 33a, 33b | + | 2a, 3a, 3b, 6a, 6b, 9a, 9b, 11a, 11b, 14a, 14b, 15a, 15b, 20a, |
| + | 20b, 21a, 21b, 27a, 27b, 28a, 28b, 29a, 29b, 30a, 30b, 31a, 33a, 33b | ||
The gel electrophoresis of the colony PCRs didn't run as expected. We have to repeat it on Monday. | The gel electrophoresis of the colony PCRs didn't run as expected. We have to repeat it on Monday. | ||
Revision as of 22:04, 24 July 2010
We picked two colonies for each the following parts
2a, 3a, 3b, 6a, 6b, 9a, 9b, 11a, 11b, 14a, 14b, 15a, 15b, 20a, 20b, 21a, 21b, 27a, 27b, 28a, 28b, 29a, 29b, 30a, 30b, 31a, 33a, 33b
The gel electrophoresis of the colony PCRs didn't run as expected. We have to repeat it on Monday. We decided anyhow the purify the plasmids tomorrow.
For the PCR we used the [pcr protocol] protocol. For the gel electrophoresis we use 1% and 2% agarose gels with [http://openwetware.org/wiki/TBE TBE buffer].
