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BIOTEC Dresden/Notepad/23 July 2010
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(Difference between revisions)
(New page: {{Biotec_Dresden/Header}} We picked two colonies for each the following parts 2a, 3a, 3b, 6a, 6b, 9a, 9b, 11a, 11b, 14a, 14b, 15a, 15b, 20a, 20b, 21a, 21b, 27a, 27b, 28a, 28b, 29a, 29b, ...) |
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For the PCR we used the [pcr protocol] protocol. | For the PCR we used the [pcr protocol] protocol. | ||
| - | For the gel electrophoresis we use 1% and 2% agarose gels with | + | For the gel electrophoresis we use 1% and 2% agarose gels with [http://openwetware.org/wiki/TBE TBE buffer]. |
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Revision as of 22:00, 24 July 2010
We picked two colonies for each the following parts
2a, 3a, 3b, 6a, 6b, 9a, 9b, 11a, 11b, 14a, 14b, 15a, 15b, 20a, 20b, 21a, 21b, 27a, 27b, 28a, 28b, 29a, 29b, 30a, 30b, 31a, 33a, 33b
The gel electrophoresis of the colony PCRs didn't run as expected. We have to repeat it on Monday. We decided anyhow the purify the plasmids tomorrow.
For the PCR we used the [pcr protocol] protocol. For the gel electrophoresis we use 1% and 2% agarose gels with [http://openwetware.org/wiki/TBE TBE buffer].
