Team:Caltech/Week 4
From 2010.igem.org
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** Since the brick is under an arabinose-inducible promoter, two versions of each solution were made - one containing arabinose, and one without arabinose. | ** Since the brick is under an arabinose-inducible promoter, two versions of each solution were made - one containing arabinose, and one without arabinose. | ||
** Pigment production was subjectively measured every hour for five hours. It seemed like there wasn't going to be much pigment produced so the solutions were left overnight in the shaker. | ** Pigment production was subjectively measured every hour for five hours. It seemed like there wasn't going to be much pigment produced so the solutions were left overnight in the shaker. | ||
+ | * Obtain the absorbance of hydrolyzed linseed oil using ethanol as blank. The peak shifts toward larger wavelength comparing to pure linseed oil. | ||
+ | |||
+ | * Test the absorbance of .25wt% agar in glycerol, .5wt% agar in glycerol and water at 632nm. .25 wt% agar gives the lowest absorbance value. | ||
==Thursday 7/8== | ==Thursday 7/8== | ||
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** [http://partsregistry.org/Part:B0015 B0015] | ** [http://partsregistry.org/Part:B0015 B0015] | ||
** [http://partsregistry.org/Part:K124017 K124017] | ** [http://partsregistry.org/Part:K124017 K124017] | ||
+ | * Background reaction using AIBN | ||
+ | ** Test both lysed and unlysed cell | ||
+ | ** Solvent: .1 ml .5 wt % agar in glycerol | ||
+ | ** Amount of AIBN: 3 wt % with respect to the cell pellet. | ||
+ | ** Reaction is run under theater light for 3 hrs. | ||
+ | ** The unlysed cell appears to be lysed at the end of 3 hrs. | ||
==Weekend 7/10-11== | ==Weekend 7/10-11== | ||
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* Transformation results: Transformations failed. | * Transformation results: Transformations failed. | ||
** There could be a problem with the antibiotic used or there could also be an issue with the digestions and/or ligations. | ** There could be a problem with the antibiotic used or there could also be an issue with the digestions and/or ligations. | ||
- | + | * Background reaction using AIBN with more controls | |
+ | ** After 3 hrs of reaction, transfer a small sample from each reaction into separate 5 ml of LB solutions and leave the solution in the shaker overnight. | ||
+ | ** The sample from the reaction with 3wt% AIBN, glycerol, and cells containing K274004 biobrick produces a slightly cloudy solution with large amount of dark green pigments precipitated at the bottom, which is similar to the sample taken from a lysed solution. | ||
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Revision as of 23:33, 16 July 2010
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Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15
Monday 7/5Note: Today was another Institute Holiday. Tuesday 7/6
Wednesday 7/7
Thursday 7/8
Friday 7/9
Weekend 7/10-11
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