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Team:Paris Liliane Bettencourt/Project/Memo-cell/Materials
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==Construction of the test for transposase== | ==Construction of the test for transposase== | ||
We have designed and then send to synthesis the different arms (wild-type and mutant) of the transposon. We then integrate between this arms the cassette for Kanamycine resistance and LacZ. Once this construction obtain we put it on the chromosome thanks to a plasmid with pir dependent origin of replication and transform the plasmid carrying Pbad Int Xis Tn916. The last part was to induce the transposase. | We have designed and then send to synthesis the different arms (wild-type and mutant) of the transposon. We then integrate between this arms the cassette for Kanamycine resistance and LacZ. Once this construction obtain we put it on the chromosome thanks to a plasmid with pir dependent origin of replication and transform the plasmid carrying Pbad Int Xis Tn916. The last part was to induce the transposase. | ||
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Revision as of 03:59, 28 October 2010
Memo-Cell project
Construction of the test for transposase
We have designed and then send to synthesis the different arms (wild-type and mutant) of the transposon. We then integrate between this arms the cassette for Kanamycine resistance and LacZ. Once this construction obtain we put it on the chromosome thanks to a plasmid with pir dependent origin of replication and transform the plasmid carrying Pbad Int Xis Tn916. The last part was to induce the transposase.
