BIOTEC Dresden/Notepad/16 September 2010
From 2010.igem.org
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Overnight cultures of the plasmids were prepared from the glycerol stocks. | Overnight cultures of the plasmids were prepared from the glycerol stocks. | ||
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+ | '''AHL Sensor''' | ||
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+ | <p>Kinetic measurement of AHL induction of GFP with part 17 in LB.</P> | ||
+ | <p>Measurement of emission spectra of GFP and RFP with the nanodrop. An uninduced culture of part 17 was used as a blank. Measurement of the spectra of parts 48 and 49 each in LB and EZ. | ||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} |
Revision as of 16:23, 27 October 2010
Fusion Protein
Restriction digest of the pure plasmids: pETMM43 and pETMMzz and gel was run to get the linearized plasmids.
Overnight cultures of the plasmids were prepared from the glycerol stocks.
AHL Sensor
Kinetic measurement of AHL induction of GFP with part 17 in LB.
Measurement of emission spectra of GFP and RFP with the nanodrop. An uninduced culture of part 17 was used as a blank. Measurement of the spectra of parts 48 and 49 each in LB and EZ.
July |
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