Team:Tokyo Tech/Project/wolf coli
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<font size="5"><b>4 Wolf coli. Overview</b></font> | <font size="5"><b>4 Wolf coli. Overview</b></font> | ||
==Introduction== | ==Introduction== | ||
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Have you heard the legend of 'The Wolfman'? They're ordinary man at daytime, but suddenly transform into a ferocious wolf in the full-moon night. Our project aim to imitate the character of Wolfman, more specifically, designing two types of E.coli that helps each other to survive at daytime, whereas competing at full moon night. | Have you heard the legend of 'The Wolfman'? They're ordinary man at daytime, but suddenly transform into a ferocious wolf in the full-moon night. Our project aim to imitate the character of Wolfman, more specifically, designing two types of E.coli that helps each other to survive at daytime, whereas competing at full moon night. | ||
- | + | [[Image:Tokyotech_wolfcoli_ver3.png|left|thumb|250px|Figure3-1. Introduction of “Wolf coli.”]] | |
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[[Image:Tokyotech wolfcoli system.png|left|thumb|300px|Figure3-2. Overview of “Wolfcoli” system]] | [[Image:Tokyotech wolfcoli system.png|left|thumb|300px|Figure3-2. Overview of “Wolfcoli” system]] |
Revision as of 15:04, 27 October 2010
4 Wolf coli. Overview
Contents |
Introduction
Have you heard the legend of 'The Wolfman'? They're ordinary man at daytime, but suddenly transform into a ferocious wolf in the full-moon night. Our project aim to imitate the character of Wolfman, more specifically, designing two types of E.coli that helps each other to survive at daytime, whereas competing at full moon night.
In order to create the “Wolf coli.”, we introduced " red-light-dependent gene expression network"[1] and "band-detect network"[2] and combined these networks with the Artificial Cooperation System (Fig. 3-2). We conducted experiment on series of OmpC promoter (PompC), and LacIM1 which are crucial parts of our networks.
BioBrick
We succeeded in designing 2 new osmoregulative promoters, PompC(CB) (BBa_K395302) and PompC(CS1) (BBa_K395303), which can be utilized in the red-light-dependent gene expression network (Fig. 3-3).
We characterized lacIM1 (BBa_K082026), a mutation of LacI (BBa_?????),which is a key component in the band-detect network. In fact, this part was registered by USTC(2008) [3], however it was not well characterized in the BioBrick registry. We found that product of lacIM1 shows weaker repression to Plac than its wild type. (Fig. 3-4)
Wolf coli. and Artificial Cooperative System
The Aftificial Cooperation System was designed to be switching off during the “full moon night” which means sympathetic coli. would be transformed into wolf coli. by induction of the “full moon light”. During this period, communication between 2 types of cells is inhibited. Therefore, both types of cells are competitors and they are unable to recognize each other by quorum sensing.
Artificial Cooperation system turns on during the daytime and the crescent-moon night which means the systems is activated by either weak or strong light intensity. In contrast, the system is off during the full moon night due the inhibition of intermediate light intensity.
POmpC in red-light-dependent gene expression network
OmpC promoter, in red-light-dependent gene expression network, plays a crucial role in initiating the transformation of sympathetic coli. into Werewolf coli. in light sensitive system. To accomplish band-detect circuit in light sensitive system, varieties of OmpC promoters were designed and characterized in order to find an appropriate strength of the promoter particularly which can be activated by the “full moon light”.
LacIMI in band-detect network
This system is accomplished by integrating the red-light-dependent gene expression network and the band-detect network (Fig. 3-2).