Team:BIOTEC Dresden/Protocols:Cocultivation assay

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Revision as of 17:02, 26 October 2010

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Set-up

Tecan plate reader

Start the machine 20 minutes in advance and heat the chamber to 37C. Put in the plate and start the measurement with the following setting:

Measure OD at 612 nm

Measure fluorescence with an excitation wavelength of 485nm and an emission wavelength of 535nm for both GFP and YFP

Shake

Repeat measurements every 5min for 3 hours

Data processing

Normalize the data by dividing all fluorescence data by the corresponding optical density

Subtract the obtained value of the reference column from the calculated relative fluorescence of the wells containing HHL

Plot the fluorescence data over the time