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Team:Paris Liliane Bettencourt/Notebook/2010/07/07/
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Revision as of 20:06, 25 October 2010
Raphaël and Théotime
DNA Gel electrophoresis (agarose 1,0% w/v)
2 µL of EtBr was added in the gel
Protocol (3 wells)
- 10 µL of minipreped DNA (Qiagen - attC) + 2 µL of loading buffer 6x
- 10 µL of minipreped DNA (Promega - attC) + 2 µL of loading buffer 6x
- 10 µL of ladder 1Kb
Migration : 50V, 1h
<a href="/wiki/Image:Electrophoresis_070710.jpg" class="image" title="Image:electrophoresis_070710.jpg"><img alt="Image:electrophoresis_070710.jpg" src="/images/7/7e/Electrophoresis_070710.jpg" width="436" height="498" border="0" /></a>
The result is not really conclusive...
Overnight culture
10mL LB
- pSUlib EX-B0015-attC-S-mRFP1-P (+Kan) 37°C
- pBad18-IntI1 (+Amp +1% glucose) 37°C
- pSWK attP-1E (+Cm +dT) 37°C
- Pi1(+dT) 37°C
- pTSA CX1 Int lambda (+Amp) 25°C
Plates in the fridge
Antoine
Preparation of plates (Amp + Kan)
