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Team:Paris Liliane Bettencourt/Project/Population counter/Microfluidics
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Aleksandra (Talk | contribs) (New page: {{Template:paris2010_2}} <p style="display:block;"> <html> <a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Population_counter"><img src="https://2010.igem.org/wiki/im...) |
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| - | <a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Population_counter"><img src="https://static.igem.org/mediawiki/2010/3/30/Popcount.png" width="75" height="75"></a><font size=4>Population counter : Microfluidics</font><a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/ | + | <p style="display:block;"> |
| - | + | <a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Population_counter"> | |
| + | <img src="https://static.igem.org/mediawiki/2010/3/30/Popcount.png" width="75" height="75" title="Population Counter"> | ||
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| + | <font size=4>Population counter : Microfluidics</font> | ||
| + | <a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/SIP"> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/4/4c/SIP.png" width="75" height="75" align=right title="SIP"> | ||
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| + | <a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Memo-cell"> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/e/e8/Memocell.png" width="75" height="75" align=right title="Memo-Cell"> | ||
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| + | <div class="submenu"> | ||
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| + | <li><a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Population_counter" target="_self">Introduction</a></li> | ||
| + | <li><a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Population_counter/Design" target="_self">Design</a></li> | ||
| + | <li><a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Population_counter/Microfluidics" target="_self">Microfluidics</a></li> | ||
| + | <li><a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Population_counter/model" target="_self">Modelling</a></li> | ||
| + | <li><a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Population_counter/Results" target="_self">Results</a></li> | ||
| + | <li><a href="https://2010.igem.org/Team:Paris_Liliane_Bettencourt/Project/Parts" target="_self">Parts</a></li> | ||
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<br><br>When using the timer, we expect the concentration of AHL to reflect the number of events, but we must take into account that AHL can accumulate in the media. This would make our timer time-dependent rather that event-depended as we want it to be. Our solution is to use a microfluidic device. | <br><br>When using the timer, we expect the concentration of AHL to reflect the number of events, but we must take into account that AHL can accumulate in the media. This would make our timer time-dependent rather that event-depended as we want it to be. Our solution is to use a microfluidic device. | ||
<br>It also allows us to better use the counter by calculating the ratio of red fluorescent to wild-type cells directly in the microfluidic device. This way we have the results on the go, without having to plate and wait for the colonies to grow. | <br>It also allows us to better use the counter by calculating the ratio of red fluorescent to wild-type cells directly in the microfluidic device. This way we have the results on the go, without having to plate and wait for the colonies to grow. | ||
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Revision as of 20:55, 23 October 2010
Population counter : Microfluidics
When using the timer, we expect the concentration of AHL to reflect the number of events, but we must take into account that AHL can accumulate in the media. This would make our timer time-dependent rather that event-depended as we want it to be. Our solution is to use a microfluidic device.
It also allows us to better use the counter by calculating the ratio of red fluorescent to wild-type cells directly in the microfluidic device. This way we have the results on the go, without having to plate and wait for the colonies to grow.
