Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/28

From 2010.igem.org

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<Sample>(temperature in annealing:℃)<br />
<Sample>(temperature in annealing:℃)<br />
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (72)
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*2 (70)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (70)
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*4 (70)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (70)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] (70)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] (63,68)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10](58)
*vector(72)
*vector(72)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
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===4:Transformation===
===4:Transformation===

Revision as of 11:42, 19 October 2010


Contents

2010/09/28

1：DNA extraction

＜Member＞
mizuki,Takahiro

＜Sample＞
PCR products

  • 1(9/16)
  • 2(9/16)
  • 3(9/17)
  • 4(9/16)
  • 5(9/16)
  • 7(9/16)

＜Protocol＞
See Protocol 4

2：DNA Ligation

＜Member＞
mio

＜Sample＞

(after digestion)

＜Protocol＞
See Protocol 3

3:PCR

<Member>
mizuki,Takahiro

<Sample>(temperature in annealing:℃)

  • 1 (72)
  • 2 (70)
  • 4 (70)
  • 5 (70)
  • 9 (63,68)
  • 10(58)
  • vector(72)

<Protocol>
See Protocol 1

4:Transformation

<Member>
nito

<Sample>

  • 7-8-10+vector
  • 2+vector

<Protocol>
See Protocol 7


5:Electrophoresis

<Member>
nito,Takahiro

<Sample>
PCR products

  • 1 (9/28)
  • 2 (9/28)
  • 4 (9/28)
  • 5 (9/28)
  • 8 (9/27)
  • 9 (9/28)
  • 10(9/28)
  • vector

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-28 17hr 31min.JPG

6：DNA extraction

＜Member＞
Takahiro

＜Sample＞
PCR products

＜Protocol＞
See Protocol 4


7：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR productions

  • 1 (8/31)
  • 2 (9/11)
  • 4L(8/26)
  • 5 (8/31)

・Digest enzyme

  • Avr2
  • Nhe1
  • spe1

＜Protocol＞
See Prptpcpl 9


8:PCR

<Member>
Mariko

<Sample>(temperature in annealing:℃)

  • 1 (72)
  • 2 (70)
  • 3 (69)
  • 4 (70)

<Protocol>
See Protocol 1

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/28"