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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/16
From 2010.igem.org
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===materials and procedure=== | ===materials and procedure=== | ||
follow the protocol(Ligation). | follow the protocol(Ligation). | ||
| + | *bcsA,B and C(E.coli). | ||
| + | *bcsA,B,C and D(A.xylium) | ||
| + | *pSB1C3 | ||
Revision as of 13:32, 18 October 2010
Contents |
PCR/pBS1C3
member
naoto and bambi75
materials,Procedure
Same on August 23th.
- 95℃3min
- 96℃1min
- 51℃1min
- 72℃3min
- 10℃∞
（96℃～72℃/30cycle）
Agarose Gels Electro-phoresis
member
Same above
Materials,Procedure
Same protocol on August 16th. PCR Product of insert is pBS1C3
Experiment:Make Agarose Gels Electro-phoresis
Member
Same above
Materials and Procedure
Same protocol on August 16th.
Medium making
member
Same above
materials and procedure
Same protocol on August 10th.
Ligation
member
Same above
materials and procedure
follow the protocol(Ligation).
- bcsA,B and C(E.coli).
- bcsA,B,C and D(A.xylium)
- pSB1C3
