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Line 348: |
Line 348: |
| The PCR products were run on a 1.2% GelRed stained agarose gel for visualisation (see picture of gel below). </li> | | The PCR products were run on a 1.2% GelRed stained agarose gel for visualisation (see picture of gel below). </li> |
| | | |
| + | <p><p> |
| | | |
| | | |
| + | <h4>Experimental Design – Primer combinations: </h4> |
| | | |
| + | <div align="center"> |
| + | <table> |
| + | <tr> |
| + | <th>Template DNA</th> |
| + | <th>Fwd Primer</th> |
| + | <th>Rvs primer</th> |
| + | </tr> |
| | | |
| | | |
| + | <tr> |
| + | <td>DNA1.2</td> |
| + | <td> F1 (AT-FWD-1)</td> |
| + | <td> R1(AT-RVS-1)</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td> DNA2.2</td> |
| + | <td>F1 (AT-FWD-1)</td> |
| + | <td> R1(AT-RVS-1)</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>DNA1.2</td> |
| + | <td> F2 (AT-BHO-F)</td> |
| + | <td> R1(AT-RVS-1)</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>DNA2.2 </td> |
| + | <td> F2 (AT-BHO-F)</td> |
| + | <td> R1(AT-RVS-1)</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td> DNA1.2</td> |
| + | <td>F3 (AT-FWD-RBS) </td> |
| + | <td> R1(AT-RVS-1)</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td> DNA2.2</td> |
| + | <td>F3 (AT-FWD-RBS)</td> |
| + | <td> R1(AT-RVS-1)</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td> SSH20 (negative control)</td> |
| + | <td>F1 (AT-FWD-1)</td> |
| + | <td> R1(AT-RVS-1)</td> |
| + | </tr> |
| + | |
| + | |
| + | </table> |
| + | </center> |
| + | <p> </div> |
| + | |
| + | |
| + | |
| + | |
| + | <li type="disc"> |
| + | A band was seen in lane 6. This lane was using the primer and DNA template combinations of DNA2.2, F3 and R1 primers <p>= SUCCESS!!! </li> |
| + | <li type="disc"> |
| + | A PCR product is seen in lane 6 – this is the DNA2.2 template amplified with the F3 (AT-FWD-RBS) and R1 (AT-RVS-1) primers <p>= SUCCESS!! </li> |
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PROJECT LAB BOOK
Welcome to the Macquarie University project lab book page!
Here you will find a day-by-day account of our triumphs and failures.
A day-by-day progress for Agrobacterium Tumefaciens Bacteriophytochrome
20th August 2010
Genomic DNA extraction