Team:Macquarie Australia/Notebook
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- | < | + | <h1><font color="#47484c">PROJECT LAB BOOK <p> |
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+ | Welcome to the Macquarie University project lab book page! <p> | ||
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+ | Here you will find a day-by-day account of our triumphs and failures.</font></h1> </hr> | ||
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+ | <center><h2> A day-by-day progress for <i> Agrobacterium Tumefaciens </i> Bacteriophytochrome </h2> </center> | ||
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+ | 20th August 2010 <p> | ||
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+ | Genomic DNA extraction <p> | ||
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+ | <i> A. tumefaciens </i> genomic DNA extracted using the Invitrogen PureLinkTM Genomic DNA Purification kit as per the manufacturer’s protocols. </li> <li type="disc"> <p> | ||
+ | The extractions were run on a GelRed stained 1% agarose gel and photo taken for visualization (see below). </li> <p> <li type="disc"> | ||
+ | A NanoDrop spectrophotometer reading was also recorded to check the quality of the extracted genomic DNA. <p> </li> <li type="disc"> | ||
+ | The extraction was successful for all A. tumefaciens cell lysate samples (labeled DNA1.1, DNA1.2, DNA2 (See figure below) <p> </li> | ||
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+ | <p><h3><i><b>A. tumefaciens </i> genomic DNA extraction agarose results: <p> </b> </h3> | ||
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+ | All four DNA samples show a smear of gDNA. Because the samples were treated with RNase there is no band indicative of RNA visible = SUCCESS! <p> | ||
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Revision as of 00:50, 14 October 2010
PROJECT LAB BOOK
Welcome to the Macquarie University project lab book page!
Here you will find a day-by-day account of our triumphs and failures.
A day-by-day progress for Agrobacterium Tumefaciens Bacteriophytochrome
20th August 2010
Genomic DNA extraction