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BIOTEC Dresden/Notepad/11 October 2010
From 2010.igem.org
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Charansam21 (Talk | contribs) (New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> '''Parts Assembly''' ...) |
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The PCR samples were run on an agarose gel and the positive clones were identified corresponding to the bands obtained as per the desired insert length. The cultures corresponding to the positive clones were left for overnight shaking. | The PCR samples were run on an agarose gel and the positive clones were identified corresponding to the bands obtained as per the desired insert length. The cultures corresponding to the positive clones were left for overnight shaking. | ||
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| + | '''Fusion Protein''' | ||
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| + | Colony PCR for the luxI + zz fusion protein and one colony was picked for the overnight culture | ||
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| + | Restriction digest of the plasmids: pSB1T3 and pSB1C3 | ||
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| + | Transformation of the plasmid pETMM43 | ||
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Revision as of 12:05, 23 October 2010
Parts Assembly
Thirty colonies were picked for the following part and colony PCR was done.
2e + 6e + 14f
The PCR samples were run on an agarose gel and the positive clones were identified corresponding to the bands obtained as per the desired insert length. The cultures corresponding to the positive clones were left for overnight shaking.
Fusion Protein
Colony PCR for the luxI + zz fusion protein and one colony was picked for the overnight culture
Restriction digest of the plasmids: pSB1T3 and pSB1C3
Transformation of the plasmid pETMM43
July |
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August |
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September |
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October |
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