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Team:Caltech/Week 12
From 2010.igem.org
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*Use a different plate of L62-1 cells (HSP-LacI-GFP), pick 3 different colonies and re-perform the HSP-LacI-GFP | *Use a different plate of L62-1 cells (HSP-LacI-GFP), pick 3 different colonies and re-perform the HSP-LacI-GFP | ||
**Only use 0 mM Glucose and 5 mM Glucose | **Only use 0 mM Glucose and 5 mM Glucose | ||
| + | *Make new digests of L54 (HSP-LacI-RBS), E0430 (EYFP), and RFP-TetR backbone | ||
| + | *Ligate L54 to L56 (Lysis-Terminator), L54 to L57 (Another Lysis-Terminator), and L54 to E0430 | ||
| + | *Transform aforementioned ligations into cells, along with L90 and L91 (both HSP-Lysis) | ||
| + | *Attempt PCR extraction and amplification of E. coli sigma 32 (HtpR) | ||
==Tuesday 8/31== | ==Tuesday 8/31== | ||
Revision as of 20:59, 31 August 2010
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People
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 Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15
Monday 8/30
Tuesday 8/31
Wednesday 9/1Thursday 9/2Friday 9/3
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