Team:Caltech/Week 11

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==Friday 8/27==
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Grow up cell cultures, redesign Glucose-GFP expression experiment, and prepare to run the new experiment for monday.
 
 
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Latest revision as of 16:05, 17 October 2010


iGEM 2010



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Monday 8/23

  • Ligate L90, L91, and L92 (overnight ligation)

Tuesday 8/24

  • Transform the ligation product from yesterday

Wednesday 8/25

  • Pick colonies from the transformed cells. Perform colony PCR and run gel on the PCR product.
    • Gel shows negative result Failed
  • Set up HSP-LacI-GFP experiment with glucose concentration (.1mM, .5mM, 1mM, 5mM)

Thursday 8/26

  • Glucose appears to not effect the GFP expression in this experiment which contradicts the result from previous experiment.

Friday 8/27

Grow up cell cultures, redesign Glucose-GFP expression experiment, and prepare to run the new experiment for monday.

 
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