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Team:Newcastle/20 August 2010
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Please refer to the [[Team:Newcastle/Gel_electrophoresis| gel electrophoresis]], [[Team:Newcastle/Gel_extraction| gel extraction]] and [[TeamNewcastleNanoDrop_Spectrophotometer| NanoDrop]] protocols. '''Vacuum manifold''' will be used. | Please refer to the [[Team:Newcastle/Gel_electrophoresis| gel electrophoresis]], [[Team:Newcastle/Gel_extraction| gel extraction]] and [[TeamNewcastleNanoDrop_Spectrophotometer| NanoDrop]] protocols. '''Vacuum manifold''' will be used. | ||
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| + | =Gel Extraction= | ||
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| + | ==Aims== | ||
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| + | To purify the PCR products of digested ''yneA'', pGFPrrnB and pSB1C3 from [[Team:Newcastle/19_August_2010|yesterday]]. | ||
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| + | ==Materials and Protocol== | ||
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| + | Please refer to [[Team:Newcastle/Gel_extraction|gel extraction]]. | ||
Revision as of 10:57, 20 August 2010
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Contents |
Aims
Following yesterday's Phusion PCR, we plan to first perform gel electrophoresis, then gel extraction, and finally nanodrop to record the level of DNA.
Materials and protocol
Please refer to the gel electrophoresis, gel extraction and NanoDrop protocols. Vacuum manifold will be used.
Gel Extraction
Aims
To purify the PCR products of digested yneA, pGFPrrnB and pSB1C3 from yesterday.
Materials and Protocol
Please refer to gel extraction.
   
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