"
Team:Tokyo Tech
From 2010.igem.org
| Line 9: | Line 9: | ||
<h2>0. Project motivation ''-What is humanity?-''</h2><br> | <h2>0. Project motivation ''-What is humanity?-''</h2><br> | ||
| - | < | + | <h3>What do you think humanity is?</h3><br> |
This is a common question which human have tried for long time. Answer might be different in each person. | This is a common question which human have tried for long time. Answer might be different in each person. | ||
| Line 16: | Line 16: | ||
Then is it really impossible for the other livings to have those?<br> | Then is it really impossible for the other livings to have those?<br> | ||
| - | <font size="4" color="# | + | <font size="4" color="#cc0033">Tokyo Tech 2010 challenged this question. We designed ''E. coli'' with humanity in order to prove that sympathetic doesn’t exist only in human!</h><br> |
| - | < | + | <h2>1. Concept -''E. coli'' with humanity-</h2><br> |
To make ''E. coli'' with humanity, we decided to engineer ''E. coli'' that behave kindly and have sympathy. Below is a behavior of the ''E. coli'' with humanity we thought. | To make ''E. coli'' with humanity, we decided to engineer ''E. coli'' that behave kindly and have sympathy. Below is a behavior of the ''E. coli'' with humanity we thought. | ||
There are two kinds of cells. In normal conditions, they are competitors for survival. While in critical conditions, such that one is dying, another cell would notice the crisis of dying cell and help her. | There are two kinds of cells. In normal conditions, they are competitors for survival. While in critical conditions, such that one is dying, another cell would notice the crisis of dying cell and help her. | ||
| Line 27: | Line 27: | ||
| - | < | + | <h2>2. Apple reporters</h2><br> |
Our ''E. coli'' with humanity gives “apple” as expression of appreciation. | Our ''E. coli'' with humanity gives “apple” as expression of appreciation. | ||
It’s really difficult to make ''E. coli'' producing real apples.However,We succeeded in synthesizing two components of apples. | It’s really difficult to make ''E. coli'' producing real apples.However,We succeeded in synthesizing two components of apples. | ||
| - | < | + | <h3>2-1. Colour -Carotenoid synthetic pathway complete!-</h3><br> |
[[Image:tokyotech_top1.JPG|250px|right]]This year, our expaned over the project of Cambridge 2009 and completed it. | [[Image:tokyotech_top1.JPG|250px|right]]This year, our expaned over the project of Cambridge 2009 and completed it. | ||
| Line 45: | Line 45: | ||
| - | < | + | <h3>2-2. Fragrance -We create Apple flavor in ''E. coli''-</h3><br> |
[[Image:tokyotech_top2.JPG|250px|right]]''E. coli'' was modfied to produces ester by addtion of 2-MeBuOH or BuOH as a substrate. | [[Image:tokyotech_top2.JPG|250px|right]]''E. coli'' was modfied to produces ester by addtion of 2-MeBuOH or BuOH as a substrate. | ||
| Line 60: | Line 60: | ||
| - | < | + | <h2>3. Artificial Cooporation System</h2><br> |
In figure1,we explain ''E. coli'' with humanity we want to create. We already explain No.4 in the figure. | In figure1,we explain ''E. coli'' with humanity we want to create. We already explain No.4 in the figure. | ||
Next move on to No.1&2&3. In ordr to realize the idea, we constructed a system that named “Artificial Cooperation System" | Next move on to No.1&2&3. In ordr to realize the idea, we constructed a system that named “Artificial Cooperation System" | ||
| Line 70: | Line 70: | ||
| - | < | + | <h3>3-1. Genetic circiut of Artificial Cooperation System</h3><br> |
There are two types of ''E. coli'' in this system. These two types of cells recognize each other by Quorum Sensing. | There are two types of ''E. coli'' in this system. These two types of cells recognize each other by Quorum Sensing. | ||
| Line 82: | Line 82: | ||
| - | < | + | <h3>3-2. mathematical modeling of Artificial Cooperation System</h3><br> |
[[Image:tokyotech_top5.JPG|250px|right]]We simulated Artificial Cooporation System and from this result, we predicted our system can work . | [[Image:tokyotech_top5.JPG|250px|right]]We simulated Artificial Cooporation System and from this result, we predicted our system can work . | ||
| Line 96: | Line 96: | ||
| - | < | + | <h3>3-3. Lux repression promoter can work!</h3><br> |
[[Image:tokyotech_top3.JPG|250px|right]]In order to construct Artificial Cooporation System, lux activation/repression device plays the key role.<br> | [[Image:tokyotech_top3.JPG|250px|right]]In order to construct Artificial Cooporation System, lux activation/repression device plays the key role.<br> | ||
| Line 108: | Line 108: | ||
| - | < | + | <h3>3-4. Antibiotic Activation Device</h3><br> |
[[Image:tokyotech_top4.JPG|250px|right]]We introduced chloramphenicol resistance gene the downstream of lux activation promoter. <br> | [[Image:tokyotech_top4.JPG|250px|right]]We introduced chloramphenicol resistance gene the downstream of lux activation promoter. <br> | ||
This device activate in the presence of AHL. Sinal molecule activates antibiotic resistance gene. | This device activate in the presence of AHL. Sinal molecule activates antibiotic resistance gene. | ||
| Line 122: | Line 122: | ||
| - | < | + | <h2>4. Full moon inhibits Artificial Cooporation system</h2><br> |
[[Image:tokyotech_top6.JPG|250px|right]]In order to have a more intelligible& imaginable system, we tried to link our project to a famous& well-known character ”Wolfman”.<br> | [[Image:tokyotech_top6.JPG|250px|right]]In order to have a more intelligible& imaginable system, we tried to link our project to a famous& well-known character ”Wolfman”.<br> | ||
| Line 134: | Line 134: | ||
| - | < | + | <h3>4-1. New series of osmoregulative promoters</h3><br> |
[[Image:tokyotech_top7.JPG|250px|left]][[Image:tokyotech_top8.JPG|250px|left]]<br> | [[Image:tokyotech_top7.JPG|250px|left]][[Image:tokyotech_top8.JPG|250px|left]]<br> | ||
| Line 149: | Line 149: | ||
| - | < | + | <h3>4-2. LacIMI showed weaker repression than WT</h3><br> |
[[Image:tokyotech_top9.JPG|250px|right]] | [[Image:tokyotech_top9.JPG|250px|right]] | ||
Revision as of 16:40, 26 October 2010
Contents |
0. Project motivation -What is humanity?-
What do you think humanity is?
This is a common question which human have tried for long time. Answer might be different in each person.
According to LONGMAN dictionary, humanity means,
“the state of being human rather than an animal or machine. For example, kindness, respect and sympathy towards others.”
Then is it really impossible for the other livings to have those?
Tokyo Tech 2010 challenged this question. We designed E. coli with humanity in order to prove that sympathetic doesn’t exist only in human!</h>
1. Concept -E. coli with humanity-
To make E. coli with humanity, we decided to engineer E. coli that behave kindly and have sympathy. Below is a behavior of the E. coli with humanity we thought. There are two kinds of cells. In normal conditions, they are competitors for survival. While in critical conditions, such that one is dying, another cell would notice the crisis of dying cell and help her. She gives him “apples” as a expression of her appeciation. The relationship like this exists in human beings.
2. Apple reporters
Our E. coli with humanity gives “apple” as expression of appreciation. It’s really difficult to make E. coli producing real apples.However,We succeeded in synthesizing two components of apples.
2-1. Colour -Carotenoid synthetic pathway complete!-
We succeeded in synthesizing zeaxanthin by adding crtZ into crtEBIY likewise astaxanthin by crtZW. We could identify them by TLC. Astaxanthin is the final metabolite of carotenoid synthetic pathway. (See more…)
2-2. Fragrance -We create Apple flavor in E. coli-
This ester gives off apple fragrance. (See more…)
3. Artificial Cooporation System
In figure1,we explain E. coli with humanity we want to create. We already explain No.4 in the figure. Next move on to No.1&2&3. In ordr to realize the idea, we constructed a system that named “Artificial Cooperation System"
3-1. Genetic circiut of Artificial Cooperation System
There are two types of E. coli in this system. These two types of cells recognize each other by Quorum Sensing. CellA & CellB carry LasI gene and LuxI gene respectively. To see the rescuing process, click on the right figure to start (See more…)
3-2. mathematical modeling of Artificial Cooperation System
(See more…)
3-3. Lux repression promoter can work!
Lux repression part has never been measured in iGEM while Lux activation part has measured by many iGEM teams. This year we assayed the activity of lux repression promoter.(See more…)
3-4. Antibiotic Activation Device
This device activate in the presence of AHL. Sinal molecule activates antibiotic resistance gene. (See more…)
4. Full moon inhibits Artificial Cooporation system
In our attempt to produce “wolfcoli”, we introduced light/swiching function to the Artificial Cooperation System. Light sensitive promoter “PompC” and synthetic band detector circuit were used to perform function designed. (See more…)
4-1. New series of osmoregulative promoters
We introduced two new series of light sensitive promoters. We also characterized the activity of lacIM1 which is necessary for werewolf System. (See more…)
4-2. LacIMI showed weaker repression than WT
</div>
</div>
