Team:Peking/Notebook/XTeng
From 2010.igem.org
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==June== | ==June== | ||
+ | ===6.7-6.13=== | ||
+ | Primer design | ||
+ | *For expression test | ||
+ | |||
+ | pET21a-MBP-His | ||
+ | |||
+ | pET 39b DsbA-MBP | ||
+ | |||
+ | pMAL p4X-MBP-MBP | ||
+ | |||
+ | pET21a-MerR-His | ||
+ | |||
+ | pET 39b DsbA-MerR | ||
+ | |||
+ | pMAL p4X-MBP-MerR | ||
+ | |||
+ | *For standard part | ||
+ | |||
+ | MBP_SD | ||
+ | |||
+ | DsbA-MBP_SD | ||
+ | |||
+ | MBP-MBP_SD | ||
+ | |||
+ | MerR_SD | ||
+ | |||
+ | DsbA-MerR_SD | ||
+ | |||
+ | MBP-MerR_SD | ||
+ | |||
+ | ===6.14-6.20=== | ||
+ | Literature Review & Experimental Design for Periplasmic MBP | ||
+ | |||
+ | |||
+ | ==July== | ||
<html> | <html> |
Revision as of 07:31, 26 October 2010
After carefully studied the structure of MerR, I constructed MerR-MBP (Metal Binding Peptide). Additionaly, I helped to construct and standardize the periplasmic metal binding peptide – DsbA-MerR-MBP using a similar strategy as MerR-MBP. After inductive expression of MerR-MBP-His6, DsbA-PbrR-MBP-His6 and DsbA-MerR-MBP-His6, I use SDS-PAGE and western blot to confirm the expression and localization of MBP and DsbA-MBP.
There are many pictures and graphs in my notes, I strongly recommand you to download them for more precise reading.
Contents |
Contents
June
6.7-6.13
Primer design
- For expression test
pET21a-MBP-His
pET 39b DsbA-MBP
pMAL p4X-MBP-MBP
pET21a-MerR-His
pET 39b DsbA-MerR
pMAL p4X-MBP-MerR
- For standard part
MBP_SD
DsbA-MBP_SD
MBP-MBP_SD
MerR_SD
DsbA-MerR_SD
MBP-MerR_SD
6.14-6.20
Literature Review & Experimental Design for Periplasmic MBP
July