Team:Peking/Notebook/XTeng
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=='''Contents'''== | =='''Contents'''== | ||
- | * <span style="font-size:4mm;">[[Team:Peking/Notebook/ | + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/XTeng#June| June, 2010]]</span> |
- | * <span style="font-size:4mm;">[[Team:Peking/Notebook/ | + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/XTeng#July| July, 2010]]</span> |
- | * <span style="font-size:4mm;">[[Team:Peking/Notebook/ | + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/XTeng#August| August, 2010]]</span> |
- | * <span style="font-size:4mm;">[[Team:Peking/Notebook/ | + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/XTeng#September| September, 2010]]</span> |
- | * <span style="font-size:4mm;">[[Team:Peking/Notebook/ | + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/XTeng#October| October, 2010]]</span> |
==June== | ==June== |
Revision as of 07:29, 26 October 2010
After carefully studied the structure of MerR, I constructed MerR-MBP (Metal Binding Peptide). Additionaly, I helped to construct and standardize the periplasmic metal binding peptide – DsbA-MerR-MBP using a similar strategy as MerR-MBP. After inductive expression of MerR-MBP-His6, DsbA-PbrR-MBP-His6 and DsbA-MerR-MBP-His6, I use SDS-PAGE and western blot to confirm the expression and localization of MBP and DsbA-MBP.
There are many pictures and graphs in my notes, I strongly recommand you to download them for more precise reading.
Contents
June