Team:Peking/Notebook/ALiu

From 2010.igem.org

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[[https://2010.igem.org/Team:Peking/Notebook/ALiu TOP]]
[[https://2010.igem.org/Team:Peking/Notebook/ALiu TOP]]
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===6.24===
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Transform plasmids to Trans5a. pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3, pSB1C3, pSB1K3, pSB1T3, pSB3C5, pSB3T5, pSB4C5, pSB4K5, pMAL-C4X, pET-39b.
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===6.26===
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Prepare plasmid DNA. pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3, pSB1C3, pSB1K3, pSB1T3, pSB3C5, pSB3T5, pSB4C5, pSB4K5, pMAL-C4X, pET-39b
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Digest the plasmid DNA with EcoRI &PstI.
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Retrieve the digested product
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===6.27===
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Transform plasmids to Trans5a.1-12O, pSD-MBD, pASK-MBD, NRI.
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===6.28===
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Picking colonies and shaking at 37℃ overnight. 1-12O
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Prepare plasmid DNA. pSD-MBD, pASK-MBD, NRI.
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===6.29===
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PCR Strain NRI with two pairs of primers
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===6.30===
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PCR Strain NRI with two pairs of primers
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Retrieve the PCR product
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Prepare plasmid DNA. 1-12O

Revision as of 03:04, 26 October 2010





   Ao Liu's Notes
                                                                                                                                                goto her page
I Conducted mutagenesis library for Mer OP and research on the feature of promoter functional sites through screening the library (characterizging promoters with different dyad MerR binding site). Then promoters with different Hg binding affinity was integrated with the results gained from operation analysis to acquire the wider range of bioreproter Hg-sensitivity.

download her notes

Contents

June

Mon Tue Wed Thu Fri Sat Sun
- 1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 - - - -

[TOP]

6.24

Transform plasmids to Trans5a. pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3, pSB1C3, pSB1K3, pSB1T3, pSB3C5, pSB3T5, pSB4C5, pSB4K5, pMAL-C4X, pET-39b.

6.26

Prepare plasmid DNA. pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3, pSB1C3, pSB1K3, pSB1T3, pSB3C5, pSB3T5, pSB4C5, pSB4K5, pMAL-C4X, pET-39b

Digest the plasmid DNA with EcoRI &PstI.

Retrieve the digested product

6.27

Transform plasmids to Trans5a.1-12O, pSD-MBD, pASK-MBD, NRI.

6.28

Picking colonies and shaking at 37℃ overnight. 1-12O

Prepare plasmid DNA. pSD-MBD, pASK-MBD, NRI.

6.29

PCR Strain NRI with two pairs of primers

6.30

PCR Strain NRI with two pairs of primers

Retrieve the PCR product

Prepare plasmid DNA. 1-12O