Team:Newcastle/18 August 2010

From 2010.igem.org

(Difference between revisions)
(Results)
(Results)
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'''Go back to our main [[Team:Newcastle/notebook| Lab book]] page'''
'''Go back to our main [[Team:Newcastle/notebook| Lab book]] page'''
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==Discussion==
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The concentration of the different ''yneA'' tubes range from 140.0 µl/ml to 456.6 µl/ml. The standard value for miniprep extraction is 150 µg/ml.
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==Conclusion==
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High concentration of ''yneA'' were obtained.
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Revision as of 01:53, 26 October 2010

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Contents

Miniprep of yneA BioBrick

Aims

The aim of the experiment is to extract yneA in the biobrick comapatible vector from 17th August, 2010 overnight culture.

Materials and Protocol

Please refer to protocols mentioned below for materials required:

Results

yneA 1 yneA 2 yneA 3 yneA 4
Concentration of DNA ng/µl 247.3 233.6 456.6 140.0

Table 1: Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.

Nanodrop1882010.jpeg Figure 1: Screenshot of yneA tube 1 Nanodrop18820102.jpeg Figure 2: Screenshot of yneA tube 2 Nanodrop18820103.jpeg Figure 3: Screenshot of yneA tube 3 Nanodrop18820104.jpeg Figure 4: Screenshot of yneA tube 4

Go back to our main Lab book page

Discussion

The concentration of the different yneA tubes range from 140.0 µl/ml to 456.6 µl/ml. The standard value for miniprep extraction is 150 µg/ml.

Conclusion

High concentration of yneA were obtained.

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