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Team:Peking/Notebook/XTeng
From 2010.igem.org
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| + | After carefully studied the structure of MerR, I constructed MerR-MBP (Metal Binding Peptide). Additionaly, I helped to construct and standardize the periplasmic metal binding peptide – DsbA-MerR-MBP using a similar strategy as MerR-MBP. After inductive expression of MerR-MBP-His6, DsbA-PbrR-MBP-His6 and DsbA-MerR-MBP-His6, I use SDS-PAGE and western blot to confirm the expression and localization of MBP and DsbA-MBP. | ||
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<a href="https://static.igem.org/mediawiki/2010/2/22/Note-xteng.pdf"><font color=#FFFFFF>download her notes</font></a> | <a href="https://static.igem.org/mediawiki/2010/2/22/Note-xteng.pdf"><font color=#FFFFFF>download her notes</font></a> | ||
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Revision as of 07:25, 26 October 2010
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Xin Teng's Notes
After carefully studied the structure of MerR, I constructed MerR-MBP (Metal Binding Peptide). Additionaly, I helped to construct and standardize the periplasmic metal binding peptide – DsbA-MerR-MBP using a similar strategy as MerR-MBP. After inductive expression of MerR-MBP-His6, DsbA-PbrR-MBP-His6 and DsbA-MerR-MBP-His6, I use SDS-PAGE and western blot to confirm the expression and localization of MBP and DsbA-MBP.
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download her notes
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<a href="https://2010.igem.org/Team:Peking/Team/XTeng">==go to her page==</a>
