"
BIOTEC Dresden/Notepad/20 October 2010
From 2010.igem.org
(Difference between revisions)
| Line 20: | Line 20: | ||
'''Fusion Protein''' | '''Fusion Protein''' | ||
| - | Miniprep of the overnight cultures was done for the colonies. | + | Miniprep of the overnight cultures was done for the colonies of the fusion protein. |
Ligation of the MBP in the backbone followed by its transformation chemically | Ligation of the MBP in the backbone followed by its transformation chemically | ||
| Line 26: | Line 26: | ||
Restriction digest was done to the pETMM43 vector followed by its gel purification. | Restriction digest was done to the pETMM43 vector followed by its gel purification. | ||
| + | '''AHL Sensor''' | ||
| + | |||
| + | Kinetics of part 17+51 was measured in both Ampicillin and Chloramphenicol. | ||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Revision as of 16:49, 27 October 2010
Parts Assembly
The digested parts were ligated into the plasmid backbone containing chloramphenicol.
52b, 52c, 53a, 53c, 54a, 54b, 54c, 55a, 55b, 55c
The ligation mix was heat inactivated at 65 degrees for 15 min. The mix was then transformed by chemical transformation method and plated for overnight growth of colonies.
Fusion Protein
Miniprep of the overnight cultures was done for the colonies of the fusion protein.
Ligation of the MBP in the backbone followed by its transformation chemically
Restriction digest was done to the pETMM43 vector followed by its gel purification.
AHL Sensor
Kinetics of part 17+51 was measured in both Ampicillin and Chloramphenicol.
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
