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BIOTEC Dresden/Notepad/20 October 2010
From 2010.igem.org
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Charansam21 (Talk | contribs) (New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> '''Parts Assembly''' ...) |
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The ligation mix was heat inactivated at 65 degrees for 15 min. The mix was then transformed by chemical transformation method and plated for overnight growth of colonies. | The ligation mix was heat inactivated at 65 degrees for 15 min. The mix was then transformed by chemical transformation method and plated for overnight growth of colonies. | ||
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| + | '''Fusion Protein''' | ||
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| + | Miniprep of the overnight cultures was done for the colonies. | ||
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| + | Ligation of the MBP in the backbone followed by its transformation chemically | ||
| + | |||
| + | Restriction digest was done to the pETMM43 vector followed by its gel purification. | ||
Revision as of 12:20, 23 October 2010
Parts Assembly
The digested parts were ligated into the plasmid backbone containing chloramphenicol.
52b, 52c, 53a, 53c, 54a, 54b, 54c, 55a, 55b, 55c
The ligation mix was heat inactivated at 65 degrees for 15 min. The mix was then transformed by chemical transformation method and plated for overnight growth of colonies.
Fusion Protein
Miniprep of the overnight cultures was done for the colonies.
Ligation of the MBP in the backbone followed by its transformation chemically
Restriction digest was done to the pETMM43 vector followed by its gel purification.
July |
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