"
BIOTEC Dresden/Notepad/15 October 2010
From 2010.igem.org
(Difference between revisions)
Charansam21 (Talk | contribs) |
|||
| Line 25: | Line 25: | ||
The ligation mix was transformed by chemical transformation method and left for 1 hour incubation after which they were plated and kept for overnight growth of colonies. | The ligation mix was transformed by chemical transformation method and left for 1 hour incubation after which they were plated and kept for overnight growth of colonies. | ||
| + | |||
| + | '''Fusion Protein''' | ||
| + | |||
| + | Transformation of the ligated parts: 34 and 35 into the chloramphenicol backbone | ||
<hr> | <hr> | ||
Revision as of 12:12, 23 October 2010
Parts Assembly
The following digested parts were ligated into part 1a backbone.
6e+14f, 6e+21f
The following parts were ligated into the RBS backbone.
4a
The following parts were ligated into the ptetR backbone.
6e+21f
The ligation mix was transformed by chemical transformation method and left for 1 hour incubation after which they were plated and kept for overnight growth of colonies.
Fusion Protein
Transformation of the ligated parts: 34 and 35 into the chloramphenicol backbone
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
