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Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/28
From 2010.igem.org
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＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *7-8-10,vector | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10],vector |
| - | *2,vector | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2],vector |
(after digestion) | (after digestion) | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ] | ||
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===3:PCR=== | ===3:PCR=== | ||
Revision as of 11:42, 19 October 2010
Contents |
2010/09/28
1：DNA extraction
＜Member＞
mizuki,Takahiro
＜Sample＞
PCR products
＜Protocol＞
See Protocol 4
2：DNA Ligation
＜Member＞
mio
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 3
3:PCR
<Member>
mizuki,Takahiro
<Sample>(temperature in annealing:℃)
- 1 (72)
- 2 (70)
- 4 (70)
- 5 (70)
- 9 (63,68)
- 10(58)
- vector(72)
<Protocol>
See Protocol 1
4:Transformation
<Member>
nito
<Sample>
- 7-8-10+vector
- 2+vector
<Protocol>
See Protocol 7
5:Electrophoresis
<Member>
nito,Takahiro
<Sample>
PCR products
- 1 (9/28)
- 2 (9/28)
- 4 (9/28)
- 5 (9/28)
- 8 (9/27)
- 9 (9/28)
- 10(9/28)
- vector
<Protocol>
See Protocol 8
<Result>
6：DNA extraction
＜Member＞
Takahiro
＜Sample＞
PCR products
＜Protocol＞
See Protocol 4
7：DNA Digestion
＜Member＞
Mariko
＜Sample, Materials＞
・PCR productions
- 1 (8/31)
- 2 (9/11)
- 4L(8/26)
- 5 (8/31)
・Digest enzyme
- Avr2
- Nhe1
- spe1
＜Protocol＞
See Prptpcpl 9
8:PCR
<Member>
Mariko
<Sample>(temperature in annealing:℃)
- 1 (72)
- 2 (70)
- 3 (69)
- 4 (70)
<Protocol>
See Protocol 1
