Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/11

From 2010.igem.org

(Difference between revisions)
(1:PCR)
(2:Digestion(1))
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<Sample><br />
<Sample><br />
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*1H:Avr2,FD Buffer
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1H]
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*2(9/6):Nhe1、M Buffer
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2](9/6)
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*4(8/30):Avr2,FD Buffer
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4](8/30)
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*5(8/30):Spe1、M Buffer
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5](8/30)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
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===3:Electrophoresis===
===3:Electrophoresis===

Revision as of 11:21, 19 October 2010


Contents

2010/09/11

1:PCR

<Member>
mio,easily


<Sample>


<Protocol>
See Protocol 2

(temperature in annealing)

  • 2:70.0℃

2:Digestion(1)

<Member>
Mariko,mizuki


<Sample>

  • 1H
  • 2(9/6)
  • 4(8/30)
  • 5(8/30)


<Protocol>
See Prptpcpl 9

3:Electrophoresis

<Member>
Mariko,mio


<Sample>

  • PCR products(9/11)
  • Digestion products(9/11)


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-08-06 20hr 02min (20).JPG

4:Electrophoresis

<Member>
mio


<Sample>
Digestion products (9/11)

  • 1
  • 2
  • 4
  • 5

<Protocol>
See Protocol 8


<Result>
Matsuura 2010-08-06 20hr 02min (22).JPG

5:DNA extraction

<Member>
mizuki,easily


<Sample>
PCR products(9/11)


<Protocol>
See Protocol 4

6:Digestion(2)

<Member>
easily


<Sample>

  • 1(9/11)


<Protocol>
See Prptpcpl 9

7:Electrophoresis

<Member>
Mariko,mizuki


<Sample>
Digestion products

  • 1(9/11)
  • 4L(8/26)
  • 8H


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-09-11 20hr 25min.JPG

8:DNA extraction

<Member>
mio,mizuki


<Sample>

  • 4(9/11)
  • 5(9/11)


<Protocol>
See Protocol 4


9:Ligation

<Member>
Mariko,mio


<Sample>

  • 4(9/11)
  • 5(9/11)


<Protocol>
See Protocol 10


10:DNA extraction

<Member>
Mariko,mio,mizuki


<Sample>

  • 1(9/11)
  • 2(9/11)


<Protocol>
See Protocol 4


12:Ligation

<Member>
Mariko


<Sample>

  • 1(9/11)
  • 2(9/11)


<Protocol>
See Protocol 10