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| + | <h4> The PCR program was set up as per the following: </h4> |
| + | <ol> |
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| + | <li> 94˚C for 2 minutes </li> |
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| + | <li>94˚C for 30 seconds </li> |
| + | <li> 60˚C for 30 seconds </li> |
| + | <li>72˚C for 2 minutes & 30 seconds</li> <P> |
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| + | (This was repeated for another 25 cycles) |
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| + | <li> 72˚C for 10 minutes </li> |
| + | <li> 4˚C to end. </ol></li> <P> |
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| + | <li type="disc"> |
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| + | Different combinations of the primers were used for the initial PCR reaction (see below ‘Experimental Design’ section following) </li> <li type="disc"> |
| + | Not all possible primer combinations were used due to limitations on the amount of polymerase enzyme available to us </li><li type="disc"> |
| + | The PCR products were run on a 1.2% GelRed stained agarose gel for visualisation (see picture of gel below). </li> |
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PROJECT LAB BOOK
Welcome to the Macquarie University project lab book page!
Here you will find a day-by-day account of our triumphs and failures.
A day-by-day progress for Agrobacterium Tumefaciens Bacteriophytochrome
20th August 2010
Genomic DNA extraction