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BIOTEC Dresden/Notepad/13 September 2010
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Charansam21 (Talk | contribs) (New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> '''Parts Assembly''' ...) |
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The ligation mix from 09.09.2010 was transformed by electroporation and plated and kept for overnight incubation. | The ligation mix from 09.09.2010 was transformed by electroporation and plated and kept for overnight incubation. | ||
| + | Sequencing of the following parts were done. | ||
| + | 4a, 9b, 14f, 20f, 21f | ||
| + | |||
| + | The sequencing of 20f was found to be as expected. | ||
'''Fusion Protein''' | '''Fusion Protein''' | ||
A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion. | A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion. | ||
| - | + | [[Category:BIOTEC Dresden/Notepad/September|September]] | |
| - | + | ||
| - | + | ||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Latest revision as of 22:41, 27 October 2010
Parts Assembly
The ligation mix from 09.09.2010 was transformed by electroporation and plated and kept for overnight incubation.
Sequencing of the following parts were done.
4a, 9b, 14f, 20f, 21f
The sequencing of 20f was found to be as expected.
Fusion Protein
A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.
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