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Team:Peking/Notebook/TZZhu
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| + | My work is to find a way to achieve optimization of preservation of bioreporter bacteria. Utilizing preservation media and room temperature water pump to achieve a glassy form of bacteria sample and preserve in fridge.The other part of my work is to test and get the data of the dryed samples to see if the method has fulfilled our goal to preserve bacteria. | ||
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| - | < | + | <img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> |
| - | + | <a href="https://static.igem.org/mediawiki/2010/5/5e/TZZ.pdf"><font color=#FFFFFF>download his notes</font></a> | |
| - | + | </html> | |
| - | + | =='''Contents'''== | |
| - | <a href="https:// | + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/TZZhu#Week 1st| Week 1st]]</span> |
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| - | </ | + | |
| - | < | + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/TZZhu#Week 2nd| Week 2nd]]</span> |
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| - | </ | + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/TZZhu#Week 3rd| Week 3rd]]</span> |
| + | |||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/TZZhu#Week 4th| Week 4th]]</span> | ||
| + | |||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/TZZhu#Week 5th| Week 5th]]</span> | ||
| + | |||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/TZZhu#Week 6th| Week 6th]]</span> | ||
| + | |||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/TZZhu#Week 7th| Week 7th]]</span> | ||
| + | |||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/TZZhu#Week 8th| Week 8th]]</span> | ||
| + | |||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/TZZhu#Week 9th| Week 9th]]</span> | ||
| + | |||
| + | * <span style="font-size:4mm;">[[Team:Peking/Notebook/TZZhu#Week 10th| Week 10th]]</span> | ||
| + | |||
| + | ===Week 1st=== | ||
| + | |||
| + | Get the vector with Psal promoter and double digest it with X,P. | ||
| + | |||
| + | Get the insert of GFP gene and double digest it with S,P | ||
| + | |||
| + | Ligase the two part together in order to cnstruct the plasmid of Psal promoter and GFP, | ||
| + | |||
| + | Transfer the plasmid into trans 5a bacteria | ||
| + | |||
| + | Get the vector with Pbad promoter and double digest it with X,P. | ||
| + | |||
| + | Get the insert of GFP gene and double digest it with S,P | ||
| + | |||
| + | Ligase the two part together in order to cnstruct the plasmid of Pbad promoter and GFP, | ||
| + | |||
| + | Transfer the plasmid into trans 5a bacteria | ||
| + | |||
| + | |||
| + | |||
| + | ===Week 2nd=== | ||
| + | |||
| + | Get the vector with T7 promoter and double digest it with X,P. | ||
| + | |||
| + | Get the insert of GFP gene and double digest it with S,P | ||
| + | |||
| + | Ligase the two part together in order to cnstruct the plasmid of T7 promoter and GFP, | ||
| + | |||
| + | Transfer the plasmid into trans 5a bacteria | ||
| + | |||
| + | |||
| + | |||
| + | ===Week 3rd=== | ||
| + | |||
| + | Make the different types of protection media with gradient of trehalose PVP. | ||
| + | |||
| + | |||
| + | |||
| + | ===Week 4th=== | ||
| + | |||
| + | Use IPTG to induce the fresh cell containing plasmid of T7 promoter and GFP gene to express GFP.] | ||
| + | |||
| + | Detect it with flow cytometry assay and enzyme-linked immunoadsordent assay. | ||
| + | |||
| + | Determine the respone curve of the cell, and the intensity of GFP expression level. | ||
| + | |||
| + | |||
| + | |||
| + | ===Week 5th=== | ||
| + | |||
| + | Transfer the cells of T7 promoter and GFP, Mer promoter and GFP into protection media and dry them in the water pump. Store in fringe in 4°C. | ||
| + | |||
| + | |||
| + | |||
| + | ===Week 6th=== | ||
| + | |||
| + | ShangHai EXPO | ||
| + | |||
| + | |||
| + | |||
| + | ===Week 7th=== | ||
| + | |||
| + | Recover the stored cells and use cytometry assay and enzyme-linked immunoadsordent assay, the same methods used in fresh cells to determine the same parameter. | ||
| + | |||
| + | |||
| + | |||
| + | ===Week 8th=== | ||
| + | |||
| + | Recover the stored cells and use cytometry assay and enzyme-linked immunoadsordent assay, the same methods used in fresh cells to determine the same parameter. | ||
| + | |||
| + | |||
| + | |||
| + | ===Week 9th=== | ||
| + | |||
| + | Recover the stored cells and use cytometry assay and enzyme-linked immunoadsordent assay, the same methods used in fresh cells to determine the same parameter. | ||
| + | |||
| + | |||
| + | |||
| + | ===Week 10th=== | ||
| + | |||
| + | Collect the figure and data achieved in the previous three weeks to see how the condition and vigor of cells change. | ||
| + | |||
| + | Determine which kind of protection media is the best. | ||
| + | |||
| + | |||
| + | |||
| + | <html> | ||
</div> | </div> | ||
| + | |||
| + | |||
<div id="project description bottom"> | <div id="project description bottom"> | ||
Latest revision as of 04:48, 27 October 2010
NOTEBOOK HOME
CountDown
Vistors From...
Tianze Zhu's Notes
Contents
Week 1st
Get the vector with Psal promoter and double digest it with X,P.
Get the insert of GFP gene and double digest it with S,P
Ligase the two part together in order to cnstruct the plasmid of Psal promoter and GFP,
Transfer the plasmid into trans 5a bacteria
Get the vector with Pbad promoter and double digest it with X,P.
Get the insert of GFP gene and double digest it with S,P
Ligase the two part together in order to cnstruct the plasmid of Pbad promoter and GFP,
Transfer the plasmid into trans 5a bacteria
Week 2nd
Get the vector with T7 promoter and double digest it with X,P.
Get the insert of GFP gene and double digest it with S,P
Ligase the two part together in order to cnstruct the plasmid of T7 promoter and GFP,
Transfer the plasmid into trans 5a bacteria
Week 3rd
Make the different types of protection media with gradient of trehalose PVP.
Week 4th
Use IPTG to induce the fresh cell containing plasmid of T7 promoter and GFP gene to express GFP.]
Detect it with flow cytometry assay and enzyme-linked immunoadsordent assay.
Determine the respone curve of the cell, and the intensity of GFP expression level.
Week 5th
Transfer the cells of T7 promoter and GFP, Mer promoter and GFP into protection media and dry them in the water pump. Store in fringe in 4°C.
Week 6th
ShangHai EXPO
Week 7th
Recover the stored cells and use cytometry assay and enzyme-linked immunoadsordent assay, the same methods used in fresh cells to determine the same parameter.
Week 8th
Recover the stored cells and use cytometry assay and enzyme-linked immunoadsordent assay, the same methods used in fresh cells to determine the same parameter.
Week 9th
Recover the stored cells and use cytometry assay and enzyme-linked immunoadsordent assay, the same methods used in fresh cells to determine the same parameter.
Week 10th
Collect the figure and data achieved in the previous three weeks to see how the condition and vigor of cells change.
Determine which kind of protection media is the best.
