Team:Peking/Notebook/Protocols
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- | <html> | + | <html><div id="Protocol Title"> |
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<font size=10><font color=#585858><font face="Franklin Gothic Demi Cond"> Protocols</font></font></font> | <font size=10><font color=#585858><font face="Franklin Gothic Demi Cond"> Protocols</font></font></font> | ||
+ | <br><br><br> <font color=#ffffff></html | ||
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+ | >[[Team:Peking/Notebook|Notebook]] > [[Team:Peking/Notebook/Protocols|Protocols]]<html></font> | ||
+ | </div> | ||
+ | |||
+ | <div id="notebook home"> | ||
+ | <div id="titlegreen"> | ||
<br><br> | <br><br> | ||
+ | <p> | ||
+ | <font size=5><b><font color=#74DF00>Protocols of Experiment Ver.2010</font></font></b> | ||
</div> | </div> | ||
+ | <div id="middlegreen"> | ||
+ | </html> | ||
+ | ==Peking 2010 team== | ||
+ | <html> | ||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/SDS&WB"><font size=3><font color=#FFFFFF>*SDS-PAGE & western blot protocol</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/7/7e/SDS-PAGE_%26_western_blot_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/SDS&W"><font size=3><font color=#FFFFFF>*SDS-PAGE & western sample preparation protocol</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/f/f8/SDS-PAGE_%26_western_sample_preparation_protocol-xteng.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/MBP"><font size=3><font color=#FFFFFF>*Protocol for chemical inducible expression of MBP & sample preparation</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/0/08/Protocol_for_chemical_inducible_expression_of_MBP_%26_sample_preparation.pdf" target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/DDDP"><font size=3><font color=#FFFFFF>*DNA Double Digestion Protocol</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/8/84/DNA_double_digestion_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/DGEP"><font size=3><font color=#FFFFFF>*DNA Gel Extraction Protocol</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/8/83/DNA_Gel_extraction_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/MP"><font size=3><font color=#FFFFFF>*Miniprep Protocol</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/1/19/Miniprep_Protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PCIEG"><font size=3><font color=#FFFFFF>*Protocol for Chemical Inducible Expression of GFP</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/0/06/Protocol_for_chemical_inducible_expression_of_GFP.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PDPRM"><font size=3><font color=#FFFFFF>*Protocol for DNA Purification from Reaction Mixture</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/b/bf/Protocol_for_DNA_purification_from_reaction_mixture.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PLIDPVD"><font size=3><font color=#FFFFFF>*Protocol for Ligation of Insert DNA into Plasmid Vector DNA</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/0/04/Protocol_for_ligation_of_insert_DNA_into.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br> | ||
+ | <font size=3><font color=#FFFFFF>*Protocol for PCR with EasyPfu DNA Polymerase Preparation for Reaction</font></font> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/d/da/Protocol_for_PCR_with_EasyPfu_DNA_Polymerase_Preparation_for_Reaction_Mixture.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/PPCCT"><font size=3><font color=#FFFFFF>*Protocol for Preparation of Competent Cells for Transformation</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/4/43/Protocol_for_preparation_of_competent_cells_for_transformation.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/SDMP"><font size=3><font color=#FFFFFF>*Site-directed Mutagenesis Protocol</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/d/d0/Site_directed_mutagenesis_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br> | ||
+ | <a href="https://2010.igem.org/Team:Peking/Notebook/Protocols/TP"><font size=3><font color=#FFFFFF>*Transformation Protocol</font></font></a> | ||
+ | <br> <a href="https://static.igem.org/mediawiki/2010/7/75/Transformation_protocol.pdf"target="blank"><img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20>download PDF version</a><br> | ||
+ | |||
+ | <br><br> | ||
+ | |||
+ | </div> | ||
<div id="notebook home"> | <div id="notebook home"> | ||
+ | <div id="titlegreen"> | ||
+ | <br><br> | ||
+ | <p> | ||
+ | <font size=5><b><font color=#74DF00>Protocols of Experiment Ver.2009</font></font></b> | ||
+ | </div> | ||
+ | |||
<div id="middlegreen"> | <div id="middlegreen"> | ||
+ | </html> | ||
+ | |||
+ | ==Peking 2009 team== | ||
+ | <html> | ||
+ | |||
- | |||
- | |||
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | <img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | ||
- | <a href="https://static.igem.org/mediawiki/2009/0/00/PKU_Miniprep_Protocol.pdf"><font size=3><font color=#FFFFFF>Miniprep Protocol</font></font></a> | + | <a href="https://static.igem.org/mediawiki/2009/0/00/PKU_Miniprep_Protocol.pdf"target="blank"><font size=3><font color=#FFFFFF>Miniprep Protocol</font></font></a> |
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | <img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | ||
- | <a href="https://static.igem.org/mediawiki/2009/8/85/PKU_DNA_Gel_extraction_protocol.pdf"><font size=3><font color=#FFFFFF>DNA Gel Extraction Protocol</font></font></a> | + | <a href="https://static.igem.org/mediawiki/2009/8/85/PKU_DNA_Gel_extraction_protocol.pdf"target="blank"><font size=3><font color=#FFFFFF>DNA Gel Extraction Protocol</font></font></a> |
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | <img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | ||
- | <a href="https://static.igem.org/mediawiki/2009/1/1d/PKU_DNA_digestion_protocol.pdf"><font size=3><font color=#FFFFFF>DNA Double Digestion Protocol</font></font></a> | + | <a href="https://static.igem.org/mediawiki/2009/1/1d/PKU_DNA_digestion_protocol.pdf"target="blank"><font size=3><font color=#FFFFFF>DNA Double Digestion Protocol</font></font></a> |
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | <img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | ||
- | <a href="https://static.igem.org/mediawiki/2009/4/46/PKU_Protocol_for_DNA_purification_from_reaction_mixture.pdf"><font size=3><font color=#FFFFFF>Protocol for DNA Purification From Reaction Mixture</font></font></a> | + | <a href="https://static.igem.org/mediawiki/2009/4/46/PKU_Protocol_for_DNA_purification_from_reaction_mixture.pdf"target="blank"><font size=3><font color=#FFFFFF>Protocol for DNA Purification From Reaction Mixture</font></font></a> |
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | <img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | ||
- | <a href="https://static.igem.org/mediawiki/2009/6/61/PKU_Protocol_for_ligation_of_insert_DNA_into_plasmid_vector_DNA.pdf"><font size=3><font color=#FFFFFF>Ligation of Inserting DNA into Plasmid Vector DNA</font></font></a> | + | <a href="https://static.igem.org/mediawiki/2009/6/61/PKU_Protocol_for_ligation_of_insert_DNA_into_plasmid_vector_DNA.pdf"target="blank"><font size=3><font color=#FFFFFF>Ligation of Inserting DNA into Plasmid Vector DNA</font></font></a> |
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | <img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | ||
- | <a href="https://static.igem.org/mediawiki/2009/d/da/PKU_Protocol_for_PCR_with_EasyPfu_DNA_Polymerase.pdf"><font size=3><font color=#FFFFFF>Protocol for PCR with EasyPfu DNA Polymerase</font></font></a> | + | <a href="https://static.igem.org/mediawiki/2009/d/da/PKU_Protocol_for_PCR_with_EasyPfu_DNA_Polymerase.pdf"target="blank"><font size=3><font color=#FFFFFF>Protocol for PCR with EasyPfu DNA Polymerase</font></font></a> |
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | <img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | ||
- | <a href="https://static.igem.org/mediawiki/2009/b/ba/PKU_Protocol_for_preparation_of_competent_cells_for_transformation. | + | <a href="https://static.igem.org/mediawiki/2009/b/ba/PKU_Protocol_for_preparation_of_competent_cells_for_transformation.pdf"target="blank"><font size=3><font color=#FFFFFF>Protocol for preparation of competent cells for transformation</font></font></a> |
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | <img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | ||
- | <a href="https://static.igem.org/mediawiki/2009/6/6f/PKU_Site-directed_mutagenesis_protocol.pdf"><font size=3><font color=#FFFFFF>Site-directed mutagenesis protocol</font></font></a> | + | <a href="https://static.igem.org/mediawiki/2009/6/6f/PKU_Site-directed_mutagenesis_protocol.pdf"target="blank"><font size=3><font color=#FFFFFF>Site-directed mutagenesis protocol</font></font></a> |
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | <img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | ||
- | <a href="https://static.igem.org/mediawiki/2009/1/11/PKU_Transformation_protocol.pdf"><font size=3><font color=#FFFFFF>Transformation Protocol</font></font></a> | + | <a href="https://static.igem.org/mediawiki/2009/1/11/PKU_Transformation_protocol.pdf"target="blank"><font size=3><font color=#FFFFFF>Transformation Protocol</font></font></a> |
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | <img src="https://static.igem.org/mediawiki/2010/9/91/PKU_Adobe_Reader_Logo.jpg" width=20> | ||
- | <a href="https://static.igem.org/mediawiki/2009/1/13/PKU_Protocol_for_chemical_inducible_expression_of_GFP.pdf"><font size=3><font color=#FFFFFF>Protocol for Chemical Inducible Expression of GFP</font></font></a> | + | <a href="https://static.igem.org/mediawiki/2009/1/13/PKU_Protocol_for_chemical_inducible_expression_of_GFP.pdf"target="blank"><font size=3><font color=#FFFFFF>Protocol for Chemical Inducible Expression of GFP</font></font></a> |
- | + | ||
</div> | </div> |
Latest revision as of 05:54, 26 October 2010
Protocols of Experiment Ver.2010
Peking 2010 team
*SDS-PAGE & western blot protocol
download PDF version
*SDS-PAGE & western sample preparation protocol
download PDF version
*Protocol for chemical inducible expression of MBP & sample preparation
download PDF version
*DNA Double Digestion Protocol
download PDF version
*DNA Gel Extraction Protocol
download PDF version
*Miniprep Protocol
download PDF version
*Protocol for Chemical Inducible Expression of GFP
download PDF version
*Protocol for DNA Purification from Reaction Mixture
download PDF version
*Protocol for Ligation of Insert DNA into Plasmid Vector DNA
download PDF version
*Protocol for PCR with EasyPfu DNA Polymerase Preparation for Reaction
download PDF version
*Protocol for Preparation of Competent Cells for Transformation
download PDF version
*Site-directed Mutagenesis Protocol
download PDF version
*Transformation Protocol
download PDF version
Protocols of Experiment Ver.2009
Peking 2009 team
Miniprep Protocol
DNA Gel Extraction Protocol
DNA Double Digestion Protocol
Protocol for DNA Purification From Reaction Mixture
Ligation of Inserting DNA into Plasmid Vector DNA
Protocol for PCR with EasyPfu DNA Polymerase
Protocol for preparation of competent cells for transformation
Site-directed mutagenesis protocol
Transformation Protocol
Protocol for Chemical Inducible Expression of GFP