"
BIOTEC Dresden/Notepad/2 September 2010
From 2010.igem.org
(Difference between revisions)
(New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> '''Fusion Protein''' ...) |
|||
| (2 intermediate revisions not shown) | |||
| Line 10: | Line 10: | ||
</html> | </html> | ||
| - | + | Five colonies for each of the following parts were picked and colony PCR was performed. | |
| - | + | 4a, 14f, 20f, 21f | |
| - | + | Positive clones were identified and the corresponding cultures were left for overnight shaking. | |
| + | Gradient PCR of primers for the parts and the chloramphenicol backbone was done and the right annealing temperature was determined. | ||
| + | |||
| + | 4a, 14f, 20f, 21f | ||
| + | |||
| + | '''Fusion Protein''' | ||
| + | |||
| + | To varify the correct insertion plasmids from positive colony PCR were sequenced. | ||
| + | [[Category:BIOTEC Dresden/Notepad/September|September]] | ||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Latest revision as of 22:25, 27 October 2010
Five colonies for each of the following parts were picked and colony PCR was performed.
4a, 14f, 20f, 21f
Positive clones were identified and the corresponding cultures were left for overnight shaking.
Gradient PCR of primers for the parts and the chloramphenicol backbone was done and the right annealing temperature was determined.
4a, 14f, 20f, 21f
Fusion Protein
To varify the correct insertion plasmids from positive colony PCR were sequenced.
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
