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BIOTEC Dresden/Notepad/18 August 2010
From 2010.igem.org
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The PCR products from the backbone amplification experiment were digested with DpnI restriction enzyme for 4 hours after which heat inactivation was performed. | The PCR products from the backbone amplification experiment were digested with DpnI restriction enzyme for 4 hours after which heat inactivation was performed. | ||
This was followed by a second purification with the pcr purification kit, by concentration determination with nanodrop (today: in the range 60-80 not much lower than before digestion) and finally by running the samples on an agarose gel (although detectable, the bands seemed rather weak and were accompanied by a smear) | This was followed by a second purification with the pcr purification kit, by concentration determination with nanodrop (today: in the range 60-80 not much lower than before digestion) and finally by running the samples on an agarose gel (although detectable, the bands seemed rather weak and were accompanied by a smear) | ||
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{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Latest revision as of 21:59, 27 October 2010
Parts Assembly
The PCR products from the backbone amplification experiment were digested with DpnI restriction enzyme for 4 hours after which heat inactivation was performed.
This was followed by a second purification with the pcr purification kit, by concentration determination with nanodrop (today: in the range 60-80 not much lower than before digestion) and finally by running the samples on an agarose gel (although detectable, the bands seemed rather weak and were accompanied by a smear)
July |
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