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Team:BIOTEC Dresden/Protocols:Cocultivation assay
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Latest revision as of 02:51, 28 October 2010
Set-up
Tecan plate reader
- Start the machine 20 minutes in advance and heat the chamber to 37°C. Put in the plate and start the measurement with the following setting:
- Measure OD at 612 nm
- Measure fluorescence with an excitation wavelength of 485nm and an emission wavelength of 535nm for both GFP and YFP
- Shake
- Repeat measurements every 5min for 3 hours
Data processing
- Normalize the data by dividing all fluorescence data by the corresponding optical density
- Subtract the obtained value of the reference column from the calculated relative fluorescence of the wells containing HHL
- Plot the fluorescence data over the time
