Team:Chiba/System 1/Evaluation subsystem

From 2010.igem.org

(Difference between revisions)

Latest revision as of 02:01, 28 October 2010

Version 1 :

Evaluation

Finally we did not complete to realize genetic double click system until now (Oct 27, 2010).

The tasks that remain are
1. Checking T7 RNAP pulse
1-1. Characterizing Lux/CI434 hybrid promoter
1-2. Confirming that there are no crosstalk between CI/CI434 and CI promoter/CI434 promoter
2. Demonstrating the double click function
3. Probably we need the tuning of each parts to behave as we want

The matters that we have already accomplished are
1. Characterizing T7/CI-OR1 hybrid promoter
There are some activative/repressible hybrid promoter in registry of standard biological parts.
Many of them are designed to have operator site between -10 and -35 promoter site or downstream of -10 site.
T7/CI-OR1 hybrid promoter is designed following with the same concept.
Lambda CI operator site (OR1) is attached to the downstream of T7 promoter. So this promoter was thought to be activated by T7 and repressed by CI. In experimental result, this promoter worked as it is. In addition, T7/CI hybrid promoter was characterized for the first time in biobrick community.

@@ Line 237: / Line 237: @@
          </body>
 <!--- 2nd tab End--->
-<!--- 3rd tab--->
-<div id="search-controls">
-		<form action="/Special:Search" id="searchform">
-		<input id="searchInput" name="search" type="text" title="Search 2010.igem.org [f]" accesskey="f" value="" />
-			 <input type='submit' name="go" class="searchButton" id="searchGoButton"	value="Go" title="Go to a page with this exact name if exists" />&nbsp;
-		       <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
-		</form>
-	</div> <!-- close search controls -->
-			<p>
-<style type="text/css">
-<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
-<html xmlns="http://www.w3.org/1999/xhtml">
-        <head>
-                <meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" />
-                <title>Free CSS Navigation Menu Designs 2 at exploding-boy.com</title>
-                <style type="text/css">
-<!--
-    body {
-        margin:0;
-        padding:0;
-        font: bold 11px/1.5em Verdana;
-}
-h2 {
-        font: bold 14px Verdana, Arial, Helvetica, sans-serif;
-        color: #000;
-        margin: 0px;
-        padding: 0px 0px 0px 15px;
-}
-img {
-border: none;
-}
-/*- Menu Tabs 8--------------------------- */
-    #tabs8 {
-      float:left;
-      width:100%;
-      font-size:100%;
-      line-height:normal;
-      background:#007500;
-      margin-top:0px;
-      }
-    #tabs8 ul {
-          margin:0;
-          padding:2px 10px 0 105px;
-          list-style:none;
-      }
-    #tabs8 li {
-      float:left;
-      display:inline;
-      margin:0;
-      padding:0;
-      margin-left:40px;
-      }
-    #tabs8 a {
-      float:left;
-      background:url("tableft10.gif") no-repeat left top;
-      margin:0;
-      padding:0 0 0 2px;
-      text-decoration:none;
-      }
-    #tabs8 a span {
-      float:left;
-      display:block;
-      background:url("tabright10.gif") no-repeat right top;
-      padding:5px 15px 4px 6px;
-      color:#FFF;
-      }
-    /* Commented Backslash Hack hides rule from IE5-Mac \*/
-    #tabs8 a span {float:none;}
-    /* End IE5-Mac hack */
-    #tabs8 a:hover span {
-      color:#FFFFFF;
-      }
-    #tabs8 a:hover {
-      background-position:0% -42px;
-      }
-    #tabs8 a:hover span {
-      background-position:100% -42px;
-      }
-      #tabs8 #current a {
-              background-position:0% -42px;
-      }
-      #tabs8 #current a span {
-              background-position:100% -42px;
-      }
--->
-</style>
-        </head>
-        <body>
-                <div id="tabs8">
-                <ul>
-                                <!-- CSS Tabs -->
-<li><a href="https://2010.igem.org/Team:Chiba/System_1/Testing_components"><span>Testing</span></a></li>
-<li><a href="https://2010.igem.org/Team:Chiba/System_1/Construction_Process"><span>Construction</span></a></li>
-<li><a href="https://2010.igem.org/Team:Chiba/System_1/Evaluation_subsystem"><span>Evaluation</span></a></li>
-<li><a href="https://2010.igem.org/Team:Chiba/System_1/Result"><span>Result</span></a></li>
-<li><a href="https://2010.igem.org/Team:Chiba/System_1/Remarks"><span>Remarks</span></a></li>
-                        </ul>
-                </div>
-        </body>
 </html>
-<!--- 3rd tab End --->
 <!--- Background Contents End --->
 <!-- -------------------------------------------------------------------------------
@@ Line 354: / Line 249: @@
 ===<font size="5">Evaluation</font>===
 -----
-Finally we did not complete to realize genetic double click system until now (Oct 27, 2010).
+<html>
+<td width="800px"><font size=2 face=verdana>
+Finally we did not complete to realize genetic double click system until now (Oct 27, 2010).<br><br>
 The tasks that remain are <br>
 . Checking T7 RNAP pulse<br>
 -1. Characterizing Lux/CI434 hybrid promoter<br>
 -2. Confirming that there are no crosstalk between CI/CI434 and CI promoter/CI434 promoter<br>
 . Demonstrating the double click function<br>
-. Probably we need the tuning of each parts to behave as we want<br>
+. Probably we need the tuning of each parts to behave as we want<br><br>
 The matters that we have already accomplished are<br>
@@ Line 369: / Line 266: @@
 T7/CI-OR1 hybrid promoter is designed following with the same concept.<br>
 Lambda CI operator site (OR1) is attached to the downstream of T7 promoter. So this promoter was thought to
-be activated by T7 and repressed by CI. In experimental result, the repression by CI was observed.
+be activated by T7 and repressed by CI. In experimental result, this promoter worked as it is.
+In addition, T7/CI hybrid promoter was characterized for the first time in biobrick community.
+<html>