Miniprep of filamentous cell part

Aims

The aim of the experiment is to prepare stocks of the plasmid DNA containing our filamentous cell part.

Materials and Protocol

Please refer to protocols mentioned below for materials required:

• Plasmid extraction protocol
• Nanodrop protocol

Results

Table 1: Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.

Figure 1: Screenshot of yneA tube 1 Figure 2: Screenshot of yneA tube 2 Figure 3: Screenshot of yneA tube 3 Figure 4: Screenshot of yneA tube 4

Discussion

The concentration of the different tubes range from 140.0 µl/ml to 456.6 µl/ml. The standard value for miniprep extraction is ~150 µg/ml.

Conclusion

High DNA concentration was obtained.

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Double digest of filamentous cell part, pSB1C3 and pGFP-rrnB

We performed double digests of the filamentous cell part and pSB1C3 using EcoRI and SpeI, ready for ligation.

We also performed double digests of the filamentous cell part and pGFP-rrnB using EcoRI and NheI, ready for ligation.

Materials and Protocol

Please refer to Restriction digests.