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BIOTEC Dresden/Notepad/21 October 2010
From 2010.igem.org
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Colony PCR of the overnight grown colonies from 20.10.2010 was performed. 18 colonies for each part were picked. The PCR samples were run on an agarose gel and positive clones were identified and kept for overnight incubation. | Colony PCR of the overnight grown colonies from 20.10.2010 was performed. 18 colonies for each part were picked. The PCR samples were run on an agarose gel and positive clones were identified and kept for overnight incubation. | ||
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| + | '''AHL Sensor''' | ||
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| + | <p>Digestion of the delivered gene sequence followed by its gel purification, then ligating it into the construct and transforming it overnight.</p> | ||
| + | <p>Overnight culture for the plate co-cultivation assay was done.</p> | ||
| + | [[Category:BIOTEC Dresden/Notepad/October|October]] | ||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Latest revision as of 23:18, 27 October 2010
Parts Assembly
Colony PCR of the overnight grown colonies from 20.10.2010 was performed. 18 colonies for each part were picked. The PCR samples were run on an agarose gel and positive clones were identified and kept for overnight incubation.
AHL Sensor
Digestion of the delivered gene sequence followed by its gel purification, then ligating it into the construct and transforming it overnight.
Overnight culture for the plate co-cultivation assay was done.
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