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BIOTEC Dresden/Notepad/22 September 2010
From 2010.igem.org
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| + | '''AHL Sensor''' | ||
| + | |||
| + | <p>Part 17 was measured in LB using a plate reader, with O.D 0.17.</p> | ||
| + | <p>Miniprep of the RFP plasmid and preparing their glycerol stocks.</p> | ||
| + | <p>Colonies were picked from parts 10, 11 and 17+51 for colony PCR. | ||
'''Fusion Protein''' | '''Fusion Protein''' | ||
PCR for the fusion parts followed by PCR purification. | PCR for the fusion parts followed by PCR purification. | ||
Restriction digest then purification of the digest | Restriction digest then purification of the digest | ||
| - | + | [[Category:BIOTEC Dresden/Notepad/September|September]] | |
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Latest revision as of 22:49, 27 October 2010
AHL Sensor
Part 17 was measured in LB using a plate reader, with O.D 0.17.
Miniprep of the RFP plasmid and preparing their glycerol stocks.
Colonies were picked from parts 10, 11 and 17+51 for colony PCR. Fusion Protein PCR for the fusion parts followed by PCR purification. Restriction digest then purification of the digest
July |
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