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BIOTEC Dresden/Notepad/4 October 2010
From 2010.igem.org
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'''Fusion Protein''' | '''Fusion Protein''' | ||
| - | Gradient PCR was done to plasmids pSB1C3 and pSB1T3 | + | Gradient PCR was done to plasmids pSB1C3 and pSB1T3 to determine the best temperature. |
| - | + | '''AHL Sensor''' | |
| + | <p>Digestion of part 51</p> | ||
| + | <p>Overnight cultures of parts 51 and 17 was prepared</p> | ||
| + | [[Category:BIOTEC Dresden/Notepad/October|October]] | ||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Latest revision as of 22:59, 27 October 2010
Parts Assembly
The sequencing results of the following parts were obtained. All the minipreps prepared were successful confirmed by the sequencing.
14f+6e, 21f+6e
Finally, the RBS was assembled with two reporter proteins (YFP and CFP).
Fusion Protein
Gradient PCR was done to plasmids pSB1C3 and pSB1T3 to determine the best temperature.
AHL Sensor
Digestion of part 51
Overnight cultures of parts 51 and 17 was prepared
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
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September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
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