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BIOTEC Dresden/Notepad/9 September 2010
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| + | '''Parts Assembly''' | ||
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| + | Eight colonies were picked for each part and colony PCR was done. | ||
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| + | 4a, 9b, 14f, 20f, 21f | ||
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| + | The PCR samples were run on an agarose gel and the bands obtained did not correspond to the desired insert length. This suggests that the ligation did not work and hence, the primers alone were amplified without the desired part amplification. | ||
Revision as of 12:11, 11 October 2010
Parts Assembly
Eight colonies were picked for each part and colony PCR was done.
4a, 9b, 14f, 20f, 21f
The PCR samples were run on an agarose gel and the bands obtained did not correspond to the desired insert length. This suggests that the ligation did not work and hence, the primers alone were amplified without the desired part amplification.
Fusion Protein
A colony PCR was performed to confirm the insertion of our fused insert into the plasmid pETMM43 or pETMMZZ. 15 clones from each plate were picked and reaction conditions optimized. No positive clones for the aiiA construct were found.
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
