Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/20
From 2010.igem.org
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Revision as of 16:05, 3 October 2010
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2010/9/20 Monday (watachin)
Experiment:Insert check Electrophoresis
Member
naoto and watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Experiment:PCR
Member
naoto and watachin
Materials and Procedure
Direct PCR of bcs genes
follow the protocol(Direct PCR)
Experiment:Plating
Member
naoto and watachin
Materials and Procedure
plated E.coli(JM109) that transformated bcs genes(A.xlinum) to LB + cam medium from LB broth
Experiment:Electrophoresis
Member
naoto and watachin
Result
Failure (I had the wrong protocol.)
Experiment:PCR
Member
naoto
Materials and Procedure
PCR of bcs genes of A.xylinum and E.coli
follow the protocol(Direct_PCR)
Experiment:Digestion
Member
watachin
Materials and Procedure
follow the protocol(Digestion)
Experiment:Electrophoresis
Member
watachin
Materials and Procedure
follow the protocol(Electrophoresis)
Experiment:DNA purification from agarose gel with QIAGEN
Member
watachin
Materials and Procedure
follow the protocol(DNA purification from agarose gel with QIAGEN)
Experiment:Ligation
Member
watachin
Materials and Procedure
follow the protocol(Ligation)
Experiment:Transformation
Member
watachin
Materials and Procedure
follow the protocol(Transformation)