Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/06

From 2010.igem.org

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===1:PCR===
===1:PCR===
<Member><br />
<Member><br />

Revision as of 12:50, 29 September 2010



Contents

1:PCR

<Member>
Hitomi,nito


<Sample>

  • 1(BBa_K208017)
  • 2(BBa_JM109)
  • 5(BBa_K208017)


<Protocol>
See Protocol 2

Tubes(Temperature in annealing)

  • 1(BBa_K208017) 71.5,72.0,72.5℃
  • 2(BBa_JM109) 71.0,71.5,72.0℃
  • 5(BBa_K208017) 70.0,70.5,71.0℃


2:DNA extraction

<Member>
Hitomi


<Protocol>
See Protocol 4


3:Electrophoresis

<Samlpe>
PCR products.

  • 1
  • 2
  • 5
  • 7
  • 8

<Protocol>
See Protocol 8


<Result>

Matsuura 2010-08-06 20hr 02min (16).JPG

4:Ligation

<Membber>
nito


<Sample>

  • 7
  • 8


<Protocol> See Protocol 3